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"CTRI Bulletin"
#4,(13),2020.

CTRI BULLETIN №4 (13) 2020

Journal Information: Read
Chief Editor: Ergeshov A.E.
Year of foundation: 2017
ISSN (Print): Browse
Publisher site: http://critub.ru
http://tb-bulletin.ru

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CONTENT

1)

Organization of TB services for children and adolescents at risk of TB: a look from the past at current issues (a literature review and a comment)

Ergeshov A.E., Ovsyankina E.S., Gubkina M.F., Panova L.V., Yukhimenko N.V.

5 READ MORE
2)

Modelling of receptor phagocytosis pathways in human pro-inflammatory macrophages

Erokhina M.V., Kurynina A.V., Shcherbakova E.A., Shaposhnikova D.A., Lepekha L.N.

15 READ MORE
3)

Tissue and cellular reactions in the lungs caused by different strains of slowly growing nontuberculous mycobacteria

Lepekha L.N., Krasnikova E.V., Makaryants N.N., Smirnova T.G., Sargsyan A.P.

27 READ MORE
4)

Risk factors for lethal outcome in patients with multidrug-resistant tuberculosis

Saenko S.S., Sterlikov S.A., Rusakova L.I.

35 READ MORE
5)

The peculiarities of diagnosis and course of the novel coronavirus infection in children and adolescents with pulmonary TB

Ergeshov A.E., Ovsyankina E.S., Gubkina M.F., Petrakova I.Yu., Panova L.V., Khiteva A.Yu., Krushinskaya E.A.

43 READ MORE
6)

Types and stages of pathological changes in the lungs in novel SARS-CoV-2 coronavirus infection

Kogan E.A., Demura S.A., Berezovsky Yu.S., Ergeshov A.E., Golukhova E.Z., Rybka M.M., Shigeev S.V.

49 READ MORE
7)

A structured interactive remote access survey in dynamic observation and differential diagnosis of broncho-obstructive diseases and community-acquired pneumonia

Shubin I.V., Mishlanov V.Yu., Koshurnikova E.P.

64 READ MORE
8)

Clinical and microbiological issues of the diagnosis of nontuberculous mycobacterioses in patients with pulmonary cavities

Karpina N.L., Asanov R.B., Shishkina E.R., Larionova E.E., Shabalina I.Yu., Ergeshov A.E.

73 READ MORE
9)

Difficulties in differential diagnosis of pulmonary mycobacteriosis caused by M. abscessus

Sargsyan A.P., Makaryants N.N., Lepekha L.N., Bagirov M.A., Chernousova L.N., Kоuklina G.M.

81 READ MORE
10)

Detection of mycobacteria by culture inoculation. Liquid media and automated systems

Sevastyanova E.V., Smirnova T.G., Larionova E.E., Chernousova L.N.

88 READ MORE
11)

To the 80th anniversary of Vladislav Yakovlevich Gergert

96  
12)

To the 60th anniversary of Mamed Adilovich Bagirov

97  

ORGANIZATION OF TB SERVICES FOR CHILDREN AND ADOLESCENTS AT RISK OF TB: A LOOK FROM THE PAST AT CURRENT ISSUES (a literature review and a comment)

Article 1.Page 5.
ARTICLE TITLE:

ORGANIZATION OF TB SERVICES FOR CHILDREN AND ADOLESCENTS AT RISK OF TB: A LOOK FROM THE PAST AT CURRENT ISSUES (a literature review and a comment)

DOI: 10.7868/S2587667820040019

AUTORS:

Ergeshov A.E.1, Ovsyankina E.S.1, Gubkina M.F.1, 2,, Panova L.V.1, Yukhimenko N.V.1

1 Central TB Research Institute, Moscow, Russia

2 N.I. Pirogov Russian National Research Medical University, Moscow, Russia

DESCRIPTION OF ARTICLE:

Submitted as of 08.07.2020

We traced the history of the organization of TB services for children and adolescents, made comparisons with modern approaches to the issue. The national policy of sanitary prophylaxis targeted at health-saving was outlined in the documents regulating compulsory routine screenings for TB, mass immunization of children with BCG, formation of risk groups. We emphasized that the prophylactic component of TB control in this group lost its priority as a voluntary consent from parents/guardians became necessary. Presently none of the regulatory documents combines medical, sanitary-hygienic, and juridical solutions for TB control in risk groups, which leads to discrepancies in its organization and implementation, especially in children. TB activities targeted at children at risk of TB should be outlined in an independent regulatory document, as it was in the past, considering age and protection of the right for health saving. A family, as a part of the community, should be oriented to compulsory adherence to sanitary and epidemiological norms and medical rules to protect children and adolescents from TB considering its infectious nature, chronic course, severity and high contagiousness, especially within the family.

The article was prepared under research topic No. 0515-2019-0016 “Personalized approaches to pulmonary TB treatment in children and adolescents”.

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KEYWORDS:

TB, children, risk groups, medical, sanitary-hygienic, juridical solutions.

FOR CORRESPONDENCE: 

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

Atadzhan E. Ergeshov, Doctor of Medical Sciences, Professor, Director

Tel.: +7 (499) 785-90-19

E-mail: cniit@ctri.ru

Elena S. Ovsyankina, Doctor of Medical Sciences, Professor, Principal Researcher, Head, Child and Adolescent Department

Tel.: +7 (499) 785-90-05

E-mail: detstvocniit@mail.ru

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

N.I. Pirogov Russian National Research Medical University

1, Ostrovityanova St., 117997, Moscow, Russia

Marina F. Gubkina, Doctor of Medical Sciences, Principal Researcher, Child and Adolescent Department,

Central TB Research Institute; Professor, Phthisiology Department, N.I. Pirogov Russian National Research

Medical University

Tel.: +7 (499) 785-90-27

E-mail: detstvocniit@mail.ru

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

Ludmila V. Panova, Doctor of Medical Sciences, Leading Researcher, Child and Adolescent Department

Tel.: +7 (499) 785-90-27

E-mail: detstvocniit@mail.ru

Natalya V. Yukhimenko, Doctor of Medical Sciences, Leading Researcher, Child and Adolescent Department

Tel.: +7 (499) 785-90-27

E-mail: detstvocniit@mail.ru

MODELLING OF RECEPTOR PHAGOCYTOSIS PATHWAYS IN HUMAN PRO-INFLAMMATORY MACROPHAGES

Article 2.Page 15.
ARTICLE TITLE:

MODELLING OF RECEPTOR PHAGOCYTOSIS PATHWAYS IN HUMAN PRO-INFLAMMATORY MACROPHAGES

DOI:10.7868/S2587667820040020

AUTORS:

Erokhina M.V.1, 2, Kurynina A.V.2, Shcherbakova E.A.1, Shaposhnikova D.A.2, Lepekha L.N.1

1 Central TB Research Institute, Moscow, Russia

2 M.V. Lomonosov Moscow State University, Moscow, Russia

DESCRIPTION OF ARTICLE:

Submitted as of 29.07.2020

To study the role of receptor phagocytosis in the formation of macrophage phenotypes is important for fundamental science, practical application, and better understanding of the development of inflammatory process in pulmonary tuberculosis. Depending on microenvironmental stimuli macrophages activate as pro-inflammatory (M1) or anti-inflammatory (M2) macrophages. It is assumed that the dynamics of changes in the M1/M2 ratio of macrophages in granulomas determines the activity of tuberculous inflammation in the lungs. For better survival in human lungs, M. tuberculosis uses poorly understood mechanisms for reprogramming M1-macrophages into M2-phenotype. This emphasizes the role of M1-macrophages in the human defence against M. tuberculosis and the need to study the plasticity mechanisms of inflammatory macrophages during phagocytosis. The aim of this study was to model phagocytosis through Fc- and Mn-receptors in proinflammatory human macrophages using a model of human monocytic cells of THP-1 line. The degree of functional activity of macrophages was assessed using latex particles conjugated with ligands to the mannose receptor (MnR) or Fc-receptors (FcRs). Our results show that FcRs-mediated phagocytosis predominates in the differentiation of THP-1 macrophages. This fact confirms the M1-type polarization. Activation of MnR-mediated phagocytosis occurs at the late stage of macrophage differentiation and indicates a shift in their polarization to M2-phenotype. It is assumed that similar processes occur in a human organism after infection and trigger tuberculous inflammation. The use of proinflammatory human macrophages and latex particles conjugated to mannan or IgG allows to create a model system to study the activation of FcR- and MnR-mediated phagocytosis in M1-macrophages. Subsequently, we’ll be able to compare the data with those processes that occur during tuberculous inflammation in vivo.

The study was conducted under research topic No. 0515-2019-0015: “The development of drug resistance of mycobacteria and somatic cells to TB drugs”.

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KEYWORDS:

proinflammatory macrophages, M1/M2-macrophages, receptor phagocytosis, macrophage plasticity, macrophage polarization, mannose receptor, Fc-receptor.

FOR CORRESPONDENCE:

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

M.V. Lomonosov Moscow State University

1, Building 12, Leninskie Gory, 119991, Moscow, Russia

Maria V. Erokhina, Doctor of Biological Sciences, Head of Cell Biology Laboratory,

Department of Pathomorphology, Cell Biology and Biochemistry, Central TB Research Institute;

Docent, Cell Biology and Histology Department, Biology Faculty,

M.V. Lomonosov Moscow State University

Tel.: +7 (499) 785-91-79

Е-mail: masha.erokhina@gmail.com

M.V. Lomonosov Moscow State University

1, Building 12, Leninskie Gory, 119991, Moscow, Russia

Anna V. Kurynina, Senior Teacher, Cell Biology and Histology Department, Biology Faculty

Tel.: +7 (499) 939-45-67

Е-mail: frasska@gmail.com

Daria A. Shaposhnikova, Second-Year Master’s Student, Cell Biology and Histology Department,

Biology Faculty

Tel.: +7 (499) 939-45-67

Е-mail: dasha-shap13@mail.ru

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

Ekaterina A. Shcherbakova, Junior Researcher, Department of Pathomorphology,

Cell Biology and Biochemistry

Tel.: +7 (499) 785-91-79

Е-mail: scherbakova_katya@yahoo.com

Larisa N. Lepekha, Doctor of Biological Sciences, Professor, Head of Department of Pathomorphology,

Cell Biology and Biochemistry

Tel.: +7 (499) 785-91-79

Е-mail: lep3@yandex.ru

TISSUE AND CELLULAR REACTIONS IN THE LUNGS CAUSED BY DIFFERENT STRAINS OF SLOWLY GROWING NONTUBERCULOUS MYCOBACTERIA

Article 3.Page 27.
ARTICLE TITLE:

TISSUE AND CELLULAR REACTIONS IN THE LUNGS CAUSED BY DIFFERENT STRAINS OF SLOWLY GROWING NONTUBERCULOUS MYCOBACTERIA

DOI: 10.7868/S2587667820040032

AUTORS:

Lepekha L.N., Krasnikova E.V., Makaryants N.N., Smirnova T.G., Sargsyan A.P.

Central TB Research Institute, Moscow, Russia

DESCRIPTION OF ARTICLE:

Submitted as of 30.08.2020

Aim: to describe tissue and cellular reactions in pulmonary nontuberculous mycobacterioses caused by different strains of nontuberculous mycobacteria (NTM) in resection samples from patients with pulmonary tuberculomas.

Materials and methods. We conducted morphological, microbiological and molecular genetic studies of resection samples from 40 patients with diagnosed pulmonary tuberculoma, which were also infected with different strains of slowly growing NTM, and 38 pulmonary tuberculoma patients without concomitant mycobacteriosis (controls).

Results. The development of nontuberculous mycobacteriosis is accompanied by severe non-tuberculous lymphoid infiltration of the pulmonary parenchyma, formation of histiocytic and histiocytic macrophage granulomas without caseous necrosis, which is especially typical for M. avium (22.5%), M. intracellulare (7.5%). Histiocytic granulomas are exposed to rapid fibrosing, which results in impaired structural homeostasis of the respiratory tract and developing pneumosclerosis, especially in the perivascular zone. Typical presentations of infections caused by M. kansasii (30%) and M. xenopi (27.5%) are lymphoid histiocytic infiltration of the walls of terminal respiratory ways and adjacent lung tissue accompanied by fibrosing, narrowing of the lumen or developing bronchoalveolar ectasia in the form of thin-wall cavities with ciliated epithelium. The obtained data can be used for differential diagnosis of pulmonary TB and pulmonary nontuberculous mycobacterioses.

The study was conducted under research topic No. 0515-2019-0015: “The development of drug resistance of mycobacteria and somatic cells to TB drugs”.

REFERENCES:
  1. Alvarez Figueroa M.V., Zyuzya Yu.R., Prokopenko A.V., Koblova L.A., Sarycheva R.M. The diagnostics of concurrent tuberculosis and mycobacteriosis in HIV infection. Tuberkulez i socialno znachimye zabolevaniya, 2015, no. 4, pp. 50–57. (In Russ.)
  2. Amansakhedov R.B., Lepekha L.N., Dmitrieva L.I., Andrievskaya I.Yu., Sigaev A.T., Makaryants N.N., Romanov V.V., Ergeshov A.E. X-ray and morphological semiotics of non-tuberculous pulmonary mycobacterioses. Journal of Radiology and Nuclear Medicine, 2018, vol. 99, no. 4, pp. 184–190. (In Russ.)
  3. Bagirov M.A., Lepekha L.N., Sadovnikova S.S., Erokhina M.V., Karpina N.L., Krasnikova E.V. Indications for surgery for pulmonary tuberculoma in the modern conditions. Tuberkulez i socialno znachimye zabolevaniya, 2018, no. 2, pp. 43–49. (In Russ.)
  4. Guntupova L.D., Borisov S.E., Solovieva I.P., Makarova M.V., Khachaturyani E.N. Mycobacterioses in phthisiopulmonology practice: a literature review and our experience. Prakticheskaya meditsina, 2011, no. 3 (51), pp. 39–50. (In Russ.)
  5. Zyuzya Yu.R., Kuzina M.G., Parkhomenko Yu.G. Mycobacterioses caused by nontuberculous mycobacteria: pathoanatomical characteristics. Abst. Coll. Of the Сonf. “Tuberkulez i sochetannye infektsii: vyzovy i perspectivy”, 2017, pp. 20–21. (In Russ.)
  6. Lepekha L.N., Berezovsky Yu.S., Semenova L.A., Mikhailovsky A.M. Pathomorphology of pulmonary tuberculosis. In: Pulmonary tuberculosis. Guidelines for physicians. Ed. by A. Ergeshov. Moscow, Galleya-Print, 2017, pp. 45–75. (In Russ.)
  7. Litvinov V.I., Makarova M.V., Krasnova M.A. Nontuberculous mycobacteria. Moscow, MNPCBT, 2008. 256 p. (In Russ.)
  8. Litvinov V.I., Dorozhkova I.R., Makarova M.V., Krasnova M.A., Freiman G.E. Isolation and identification of nontuberculous mycobacteria. Annals of the Russian academy of medical sciences, 2010, no. 3, pp. 7–10. (In Russ.)
  9. Mikhailovsky A.M., Churkin S.A., Pashkova N.A., Lepekha L.N. The frequency of detection and the morphological features of nontuberculous mycobacteriosis in patients with late-stage HIV infection (according to the data from Orenburg region). Tuberculosis and Lung Diseases, 2016, vol. 94, no. 12, pp. 57–61. (In Russ.)
  10. Panteleev A.M., Nikulina O.V., Khristusev A.S., Dracheva M.S., Sokolova O.S., Zonova A.V. Differential diagnosis of TB and mycobacteriosis in HIV patients. Tuberculosis and Lung Diseases, 2017, vol. 95, no. 10, 47–51. (In Russ.)
  11. Samsonova M.V., Chernyaev A.L. Granulomatous lung diseases. Pulmonologiya, 2017, vol. 27, no. 2, 250–261. (In Russ.)
  12. Ergeshov A.E., Shmelev E.I., Kovalevskaya M.N., Larionova E.E., Chernousova L.N. Nontuberculous mycobacteria in patients with pulmonary diseases (clinical and laboratory study). Pulmonologiya, 2016, vol. 26, no. 3, pp. 303–308. (In Russ.)
  13. Farhi D.C. Pathologic findings in disseminated Mycobacterium avium-intracellulare infection. A report of 11 cases. D.C. Farhi, U.D. Mason, C.R. Horsburg.Amer. J. Clin. Path., 1986, vol. 85, pp. 67–72.
  14. Piersimoni C., Daley C. Epidemiology of human pulmonary infection with nontuberculous mycobacteria. Clin. Chest Med., 2002, vol. 23, pp. 553–567.
  15. Tabarsi P., Baghael P., Farmia P. et al. Nontuberculous mycobacteria among patients who are suspected for multidrug-resistant tuberculosis – need for earlier identification of nontuberculous mycobacteria. Am. J. Med. Sci., 2009, vol. 337, no. 3, pp. 182–184.
  16. Wolinsky E. Nontuberculous mycobacteria and associated diseases. Am. Rev. Respir. Dis., 1979, vol. 119, pp. 107–159.
KEYWORDS:

nontuberculous mycobacteriosis, TB, morphology, microbiology, resection samples of the lungs.

FOR CORRESPONDENCE:

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

Larisa N. Lepekha, Doctor of Biological Sciences, Professor, Head, Department of Pathomorphology,

Cell Biology and Biochemistry

Tel.: +7 (499) 785‑91‑79

Е-mail: lep3@yandex.ru

Elena V. Krasnikova, Doctor of Medical Sciences, Senior Researcher, Surgery Department

Tel.: +7 (499) 785‑91‑96

Е-mail: el.krasn@gmail.com

Natalia N. Makaryants, Doctor of Medical Sciences, Leading Researcher, Department of Differential

Diagnosis of TB and Extracorporal Treatments

Tel.: +7 (499) 785‑91‑56

E-mail: roman4000@yandex.ru

Tatyana G. Smirnova, Candidate of Medical Sciences, Senior Researcher, Microbiology Department

Tel.: +7 (499) 785‑90‑91

E-mail: s_tatka@mail.ru

Anna P. Sargsyan, Postgraduate, Department of Differential Diagnosis of pulmonary TB and Extracorporal Treatments

Tel.: +7 (909) 248‑23‑50

E-mail: a.sargsyan1993@mail.ru

RISK FACTORS FOR LETHAL OUTCOME IN PATIENTS WITH MULTIDRUG-RESISTANT TUBERCULOSIS

Article 4.Page 35.
ARTICLE TITLE:

RISK FACTORS FOR LETHAL OUTCOME IN PATIENTS WITH MULTIDRUG-RESISTANT TUBERCULOSIS

DOI: 10.7868/S2587667820040044

AUTORS:

Saenko S.S.1, Sterlikov S.A.2, 3, Rusakova L.I.3

1 Regional Clinical Center of Phthisiopulmonology, Rostov-on-Don, Russia

2 Central Research Institute for Health Organization and Informatics, Moscow, Russia

3 Central TB Research Institute, Moscow, Russia

DESCRIPTION OF ARTICLE:

Submitted as of 07.06.2020

Objective: to study predictors of mortality in a representative sample of patients on treatment for drug resistant TB.

Materials and methods. Multicenter retrospective cohort study of 1909 cases on treatment with confirmed resistance to at least rifampicin notified in 2016. We studied the following factors: drug resistance profile (rifampicin, isoniazid, aminoglycoside or polypeptide, fluoroquinolones), treatment default, gender, age, place of residence (city or rural area), HIV status, microscopy results and lung tissue destruction at time of notification. We conducted uni- and multivariate analyses with the calculation of adjusted odds ratio (aOR).

Results. The most significant predictors of mortality were HIV infection (aOR = 5.6), treatment default (aOR = 3.9), positive sputum smear (aOR = 2.0), drug resistance (aOR = 1.3, increasing gradation from resistance to only rifampicin, only isoniazid and rifampicin, resistance to isoniazid, rifampicin, aminoglycosides/polypeptides, resistance to isoniazid, rifampicin, fluoroquinolones, to extensive drug resistance). Other significant factors were rural residence (aOR = 1.6), lung tissue destruction (aOR = 1.6), male gender (aOR = 1.3), age (aOR = 1.03 per life year).

Conclusion. The study results can be used to identify patients at risk in order to prevent lethal outcome.

REFERENCES:
  1. Sterlikov S.A., Nechaeva O.B., Burykhin V.S., Ponomarev S.B., Rusakova L.I., Dergachev A.V., Mayina N.S., Obukhova O.V. Sectoral and economic indicators of TB control in 2017-2018: Statistical data. Moscow: RIO CNIIOIZ, 2019, 59 p. (In Russ.) ISBN: 978-5-94116-027-3
  2. Bhering M., Kritski A., Nunes C., Duarte R. Multidrugresistant tuberculosis in Lisbon: unfavourable treatment and associated factors, 2000–2014. The International Journal of Tuberculosis and Lung Disease, 2019, vol. 23, no. 10, pp. 1075-108. DOI: 10.5588/ijtld.18.0596
  3. Cox V., Cox H., Pai M., Stillo J., Citro B., Brigden G. Health care gaps in the global burden of drug-resistant tuberculosis. The International Journal of Tuberculosis and Lung Disease, 2019, vol. 23, no. 2, pp. 125–135. DOI: 10.5588/ijtld.18.0866
  4. Gayoso R., Dalcolmo M., Braga J.U., Barreira D. Predictors of mortality in multidrug-resistant tuberculosis patients from Brazilian reference centers, 2005 to 2012. Brazilian Journal of Infectious Diseases, 2018, vol. 22, no. 4, pp. 305-310. DOI: 10.1016/j.bjid.2018.07.002
  5. Global tuberculosis report 2019. WHO. WHO/CDS/TB/2019.15. ISBN 978-92-4-156571-4
  6. Hood G., Trieu L., Ahuja S.D. Mortality among tuberculosis patients in New York City. The International Journal of Tuberculosis and Lung Disease, 2019, vol. 23, no. 2, pp. 252–259. DOI: 10.5588/ijtld.18.0305
  7. Huerga H., Bastard M., Kamene M., Wanjala S., Arnold A., Oucho N., Chikwanha I., Varaine F. Outcomes from the first multidrug-resistant tuberculosis programme in Kenya. The International Journal of Tuberculosis and Lung Disease, 2017, vol. 21, no. 3, pp. 314–319. DOI: 10.5588/ijtld.16.0661
  8. Kliiman K. Altraja A. Predictors and mortality associated with treatment default in pulmonary tuberculosis. The International Journal of Tuberculosis and Lung Disease, 2010, no. 14, pp. 454–463.
  9. Makhmudova M., Maxsumova Z., Rajabzoda A., Makhmadov A., van den Hof S., Mirtskhulava V. Risk factors for unfavourable treatment outcomes among rifampicin-resistant tuberculosis patients in Tajikistan. The International Journal of Tuberculosis and Lung Disease, 2019, vol. 23, no. 3, pp. 331-336. DOI: 10.5588/ijtld.18.0311
  10. Mibei D.J., Kiarie J.W., Wairia A., Kamene M., Okumu M.E. Treatment outcomes of drug-resistant tuberculosis patients in Kenya. The International Journal of Tuberculosis and Lung Disease, 2017, vol. 20, no. 11, pp. 1477-1482. DOI: 10.5588/ijtld.15.0915
  11. Nair D., Velayutham B., Kannan T., Tripathy J.P., Harries A.D., Natrajan M., Swaminathan S. Predictors of unfavourable treatment outcome in patients with multidrug-resistant tuberculosis in India. Public Health Action, 2016, vol. 7, pp. 32–38. DOI: 10.5588/pha.16.0055.
  12. Nkurunziza J., Karstaedt A.S., Louw R., Padanilam X. Treatment outcomes of pre- and extensively drugresistant tuberculosis in Johannesburg, South Africa. The International Journal of Tuberculosis and Lung Disease, 2018, vol. 22, no. 12, pp. 1469–1474. DOI: 10.5588/ijtld.18.0205
  13. Obregón G., Zevallos K., Alarcón V., Puyén Z.M., Chávez Inagaki O., Mendoza-Ticona A., Alarcón-Arrascue E., Heldal E., Moore D.A.J. Rapid drug susceptibility testing and treatment outcomes for multidrug-resistant tuberculosis in Peru. The International Journal of Tuberculosis and Lung Disease, 2018, vol. 22, no. 11, pp. 1350–1357(8). DOI: 10.5588/ijtld.17.0894
  14. Orofino R.L., Americano do Brasil P.E., Trajman A., Schmaltz C.A.S., Dalcolmo M., Rolla V.C. Predictors of tuberculosis treatment outcomes. Jornal Brasileiro de Pneumologia, 2012, vol. 38, no. 1. pp. 88–97. DOI: 10.1590/S1806-37132012000100013
  15. Osei E., Oppong S., Adanfo D., Doepe B.A., Owusu A., Kupour A.G., Der J. Reflecting on tuberculosis case notification and treatment outcomes in the Volta region of Ghana: a retrospective pool analysis of a multicentre cohort from 2013 to 2017. Global Health Resources Policy, 2019, no. 4, pp. 4–37. DOI: 10.1186/s41256-019-0128-9
  16. 16. Waitt C.J., Squire S.B. A systematic review of risk factors for death in adults during and after tuberculosis treatment. The International Journal of Tuberculosis and Lung Disease, 2011, no. 15, pp. 871–885. DOI: 10.5588/ijtld.10.0352
KEYWORDS:

multidrug-resistant tuberculosis, MDR-TB, predictors of mortality, mortality among TB patients.

FOR CORRESPONDENCE:

Regional Clinical Center of Phthisiopulmonology

24, Orskaya St., 344065, Rostov-on-Don, Russia

Sergey S. Saenko, Head, Organization and Methodology Department

Tel.: +7 (938) 110-77-77

E-mail: saenkosergey@yandex.ru

Central Research Institute for Health Organization and Informatics 11, Dobrolyubov St., 127254, Moscow, Russia

Sergey A. Sterlikov, Doctor of Medical Sciences, Deputy Director, Federal TB Monitoring Center in the Russian Federation, Programme Monitoring

Tel.: +7 (925) 507-82-21

Е-mail: sterlikov@list.ru

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

Larisa I. Rusakova, Doctor of Medical Sciences, Head, Research Administration Department

Tel.: +7 (926) 584-17-79

Е-mail: larisa.rusakova@mail.ru

THE PECULIARITIES OF DIAGNOSIS AND COURSE OF THE NOVEL CORONAVIRUS INFECTION IN CHILDREN AND ADOLESCENTS WITH PULMONARY TB

Article 5.Page 43.
ARTICLE TITLE:

THE PECULIARITIES OF DIAGNOSIS AND COURSE OF THE NOVEL CORONAVIRUS INFECTION IN CHILDREN AND ADOLESCENTS WITH PULMONARY TB

DOI: 10.7868/S2587667820040056

AUTORS:

Ergeshov A.E., Ovsyankina E.S., Gubkina M.F., Petrakova I.Yu., Panova L.V., Khiteva A.Yu., Krushinskaya E.A.

Central TB Research Institute, Moscow, Russia

DESCRIPTION OF ARTICLE:

Submitted as of 18.09.2020

We analyzed diagnosis and course of the novel coronavirus infection in 53 children and adolescents on treatment for pulmonary TB. We established that the infection quickly spread in enclosed groups (86.8% of patients contracted coronavirus). At the same time, most of patients (41 – 89.1%) had asymptomatic or subclinical course of the disease. The clinical symptoms did not differ from those in other respiratory viral infections, which did not let us suspect the coronavirus infection. In order to diagnose COVID-19 in enclosed groups it was vital to perform smear studies for SARS-CoV-2 RNA and blood studies for specific antibodies (IgG) to SARS-CoV-2 after the lockdown, as the presence of SARS-CoV-2 RNA in nasal or pharyngeal swabs allowed to establish the diagnosis in 45.7% of patients; in the other patients (54.3%) we could presume the past infection by only the detection of IgG in blood. The moderate course of infection (10.9%) was diagnosed by the detection of typical changes in the lungs at routine chest computed tomography screening during the lockdown and was not accompanied by clinical presentations of a viral infection (or accompanied by minimal presentations without changes in patients’ general condition). The novel coronavirus infection did not influence the course of TB; the progression of TB process was observed in none of patients; there were no reasons to prolong TB treatment. Generally, TB/coronavirus co-infection did not cause new complications.

The article was prepared under research topic No. 0515-2019-0016 “Personalized approaches to pulmonary TB treatment in children and adolescents”.

REFERENCES:
  1. “Asthma can be a protective factor in the coronavirus infection”. Alexander Karaulov, Academician of the
  2. Russian Academy of Sciences – about the difference in immunities, the disease course in allergic individuals and the risk of a second wave. September 14, 2020. (In Russ.) Mode of access: https://iz.ru/rubric/nauka (date of referral: 21.09.2020).
  3. Veselova E.I., Russkikh A.E., Kaminsky G.D., Lovacheva O.V., Samoylova A.G., Vasilyeva I.A. The novel coronavirus infection. Tuberculosis and Lung Diseases, 2020, vol. 98, no. 4, pp. 6–14. (In Russ.)
  4. Zvereva N.N., Saifullin M.A., Rtishchev A.Yu., Shamsheva O.V., Pshenichnaya N.Yu. The coronavirus infection in children. Pediatriya, 2020, vol. 99, no. 2, pp. 270–278. (In Russ.)
  5. The materials of the portal “Scientific Russia”. April 30, 2020. Alexander Karaulov – about mysteries of the COVID‑19 pathogenesis. (In Russ.) Mode of access: https://scientificrussia.ru/articles/aleksandr-karaulovo-zagadkah-patogeneza-covid‑19 (date of referral: 21.09.2020).
KEYWORDS:

TB, children, adolescents, coronavirus infection.

FOR CORRESPONDENCE:

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

Atadzhan E. Ergeshov, Doctor of Medical Sciences, Professor, Director

Tel.: +7 (499) 785‑90‑19

E-mail: cniit@ctri.ru

Elena S. Ovsyankina, Doctor of Medical Sciences, Professor, Principal Researcher, Head, Child and Adolescent Department

Tel.: +7 (499) 785‑90‑05

E-mail: detstvocniit@mail.ru

Marina F. Gubkina, Doctor of Medical Sciences, Principal Researcher, Child and Adolescent Department

Tel.: +7 (499) 785‑90‑27

E-mail: detstvocniit@mail.ru

Irina Yu. Petrakova, Candidate of Medical Sciences, Head, Junior Unit, Child and Adolescent Department

Tel.: +7 (499) 785‑90‑27

Е-mail: detstvocniit@mail.ru

Ludmila V. Panova, Doctor of Medical Sciences, Leading Researcher, Child and Adolescent Department

Tel.: +7 (499) 785‑90‑27

E-mail: detstvocniit@mail.ru

Antonina Yu. Khiteva, Candidate of Medical Sciences, Junior Researcher, Child and Adolescent

Department

Tel.: +7 (499) 785‑90‑05

E-mail: detstvocniit@mail.ru

Ekaterina A. Krushinskaya, Junior Researcher, Child and Adolescent Department

Tel.: +7 (499) 785‑90‑05

Е-mail: detstvocniit@mail.ru

TYPES AND STAGES OF PATHOLOGICAL CHANGES IN THE LUNGS IN NOVEL SARS-COV-2 CORONAVIRUS INFECTION

Article 6.Page 49.
ARTICLE TITLE:

TYPES AND STAGES OF PATHOLOGICAL CHANGES IN THE LUNGS IN NOVEL SARS-COV-2 CORONAVIRUS INFECTION

DOI: 10.7868/S2587667820040068

AUTORS:

Kogan E.A.1, Demura S.A.1, Berezovsky Yu.S.2, Ergeshov A.E.2, Golukhova E.Z.3, 4, Rybka M.M.3, 4, Shigeev S.V.5

1 I.M. Sechenov 1st Moscow State Medical University, Moscow, Russia

2 Central TB Research Institute, Moscow, Russia

3 A.N. Bakulev National Medical Research Center of Cardiovascular Surgery, Moscow, Russia

4 Institute of Top-Qualification Personnel Professional Training, A.N. Bakulev National Medical Research Center of Cardiovascular Surgery, Moscow, Russia

5 Bureau of Forensic Medical Examination, Moscow, Russia

DESCRIPTION OF ARTICLE:

Submitted as of 24.09.2020

Resected lung tissue from 232 patients with COVID-19 confirmed by PCR during life and/or by examining paraffin blocks of lung tissue were studied. Conducted macro-, microscopic and immunohistochemical analysis (CD3, CD20, TLR4, TLR9, Ki67, p63, OCT4, ALDH1) revealed the particularities of pathological changes in the lungs. The obtained results allow hypothesizing that such pathological processes in the lungs as diffuse alveolar damage (DAD), lymphocytic alveolitis with concomitant vasculitis of medium- and small-calibre pulmonary arterial branches, viral-bacterial pneumonia, disseminated coagulopathy with thrombosis or thromboembolism of the pulmonary artery, interstitial fibrosis, and disregeneration changes in the lung epithelium are all interrelated and may be forms-phases of lung pathology in COVID-19. The process in the lungs can develop along three morphogenetic pathways, starting with the development of diffuse alveolar damage, or lymphocytic alveolitis, or coagulopathy. The latter variant of the onset of infection is most likely associated with a primary intestinal lesion. Wherein lung damage is secondary, and develops after viremia, disseminated intravascular coagulation or cytokine storm. Squamous metaplasia with development of spheroid formations in the lungs is dysregenerative in nature, and in the future, the epithelium of such foci can undergo malignant transformation.

REFERENCES:
  1. Zairatyani O.V., Samsonova M.V., Mikhaleva L.M. et al. Atlas: pathological anatomy of the lungs in COVID‑ Moscow, 2020, 52 p. (In Russ.)
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  20. Coronavirus disease 2019 (COVID‑19). Situation report – 51. World Health Organization. Mode of access: https://www.who.int/docs/default-source/coronaviruse/situation-reports/20200311-sitrep‑51-covid‑pdf?sfvrsn=1ba62e57 10 (date of referral: 15.06.20).
  21. Xiong Y., Sun D., Liu Y. et al. Clinical and high-resolution CT features of the COVID‑19 infection: comparison of the initial and follow-up changes. Radiol., 2020, vol. 55, no. 6, pp. 332–339. DOI: https://doi.org/10.1097/RLI.0000000000000674
  22. Zhao D., Yao F., Wang L. et al. A comparative study on the clinical features of COVID‑19 pneumonia to other pneumonias. Clin. Infect. Dis., 2020, vol. 71, no. 15, pp. 756–761. DOI: https://doi.org/10.1093/cid/ciaa247
KEYWORDS:

COVID-19 infection, pathogenesis, types and stages of lung pathology.

FOR CORRESPONDENCE:

I.M. Sechenov 1st Moscow State Medical University

2, Bolshaya Pirogovskaya St., 119991, Moscow, Russia

Evgenia A. Kogan, Doctor of Medical Sciences, Corresponding Member of the Russian Academy

of Natural Sciences, Head, Department of Pathological Anatomy named after A.I. Strukov

Tel.: +7 (926) 533‑12‑71

Е-mail: koganevg@gmail.com

Sofia A. Demura, Candidate of Medical Sciences, Docent, Department of Pathological Anatomy

named after A.I. Strukov

Tel.: +7 (926) 217‑06‑34

Е-mail: sarah3618@gmail.com

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

Yury S. Berezovsky, Head, Department of Pathological Anatomy

Tel. +7 (499) 785‑91‑79

Е-mail: report-q@yandex.ru

Atadzhan E. Ergeshov, Doctor of Medical Sciences, Professor, Director

Tel.: +7 (499) 785‑90‑19

Е-mail: cniit@ctri.ru

A.N. Bakulev National Medical Research Center of Cardiovascular Surgery, Institute of Top-Qualification Personnel Professional Training

135, Rublevskoe Highway, 121552, Moscow, Russia

Elena Z. Golukhova, Academician of the Russian Academy of Sciences, Doctor of Medical Sciences,

Professor, Acting Director, Head of Cardiology and Functional Diagnosis Department

Tel.: +7 (495) 414‑77‑02

Е-mail: info@bakulev.ru

Mihail M. Rybka, Doctor of Medical Sciences, Associate Director for Anesthesiology and Intensive care, Head of the Department for Anesthesiology and Intensive care. Professor, Anesthesiology and Reanimatology Department with a course of clinical laboratory diagnostics of professional education

Tel.: +7 (495) 414‑77‑02

Е-mail: mmrybka@bakulev.ru

Bureau of Forensic Medical Examination

3, Tarny Proezd, 115201, Moscow, Russia

Sergey V. Shigeev, Doctor of Medical Sciences, Professor, Director

Tel.: +7 (985) 766‑86‑06

Е-mail: shigeevsv@zdrav.mos.ru

A STRUCTURED INTERACTIVE REMOTE ACCESS SURVEY IN DYNAMIC OBSERVATION AND DIFFERENTIAL DIAGNOSIS OF BRONCHO-OBSTRUCTIVE DISEASES AND COMMUNITY-ACQUIRED PNEUMONIA

Article 7.Page 64.
ARTICLE TITLE:

A STRUCTURED INTERACTIVE REMOTE ACCESS SURVEY IN DYNAMIC OBSERVATION AND DIFFERENTIAL DIAGNOSIS OF BRONCHO-OBSTRUCTIVE DISEASES AND COMMUNITY-ACQUIRED PNEUMONIA

DOI: 10.7868/S258766782004007X

AUTORS:

Shubin I.V.1, Mishlanov V.Yu.2, Koshurnikova E.P.2

1 Change Implementation Centre at the Ministry of Health of Moscow Region, Krasnogorsk, Russia

2 Perm State Medical University named after E.A. Wagner, Perm, Russia

DESCRIPTION OF ARTICLE:

Submitted as of 06.07.2020

The aim of the study was to implement a remote access survey using the respiratory module of the interactive automated “Electronic polyclinic” questionnaire for differential diagnosis and dynamic observation of patients with chronic obstructive pulmonary disease (COPD), bronchial asthma (BA), community-acquired pneumonia (CAP). Totally 219 patients (30 with severe COPD, 28 with severe BA, 41 with CAP and 120 healthy individuals) completed the questionnaire. The statistical analysis of the obtained data used the software package Statistica 10.0. According to the automated conclusion of the computer programme “Electronic policlinic”, patients with CAP had the following syndromes: pulmonary-pleural syndrome, fever, respiratory failure, bronchitis. Patients with BA commonly had atopic and broncho-obstructive syndromes. Patients with COPD suffered from broncho-obstructive syndrome, bronchitis and respiratory failure. The detailed study of cough and dyspnea allowed to develop specific patterns of BA, COPD and CAP, which, together with syndrome diagnosing, could be used for diagnosis, differential diagnosis, effective observation of disease course, and disease control. The implementation of a remote access survey allows to carry out dynamic observation of symptoms, which results in timely administration of diagnostic studies and treatment correction, thus improving treatment outcomes and quality of life.

REFERENCES:
  1. Bekker K.N., Mishlanov V.Yu., Katkova A.V., Koshurnikova E.P., Syromyatnikova L.I. Cardiovascular disease morbidity rate in patients with different chronic obstructive pulmonary disease phenotypes. The Bulletin of Contemporary Clinical Medicine, 2019, vol. 12, issue 1, pp. 24–30. (In Russ.)
  2. Bekker K.N., Mishlanov V.Yu., Koshurnikova E.P., Katkova A.V. The algorithm for optimization of dynamic observation of patients with combined course of COPD and cardiovascular diseases based on data from the regional electronic patient register. Uralsky meditsinsky zhurnal, 2019, no. 4, pp. 75–81. (In Russ.)
  3. The high-risk group for COVID‑19 [Electronic resource] http://admhmansy.ru/news/3668/149565 (Date of referral: 01.07.2020)
  4. Kochetov A.G., Lyang O.V., Masenko V.P., Zhirov I.V., Nakonechnikov S.N., Tereshchenko S.N. The methods of statistical processing of medical data: Methodical recommendations for resident doctors, medical postgraduates and researchers. Moscow, 2012, 42 p. (In Russ.)
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  6. Mishlanov V.Yu., Katkova A.V., Dugina A.A., Kuznetsova V.D., Tepanyan A.T., Zhigulev A.N. The automated system for diagnostics of gastrointestinal syndromes: “Electronic polyclinic”. Eksperimentalnaya i klinicheskaya gastroenterologiya, 2016, no. 11, pp. 114–118. (In Russ.)
  7. Mishlanov V.Yu., Mishlanov Ya.V., Katkova A.V., Bolshakova E.S., Shapenkova A.S. The interactive patient questionnaire and the development of automated systems to aid general practitioners in diagnosing respiratory diseases. Prakticheskaya pulmonologiya, 2016, no. 1, pp. 24–29. (In Russ.)
  8. Nikitin A.E., Chereshnev V.A., Mishlanov V.Yu., Koshurnikova E.P., Shubin I.V., Katkova A.V., Zalaeva A.B. et al. The peculiarities of echocardiographic changes in patients with chronic obstructive pulmonary disease and arterial hypertension. Klinicheskaya meditsina, 2018, vol. 96, no. 12, pp. 1088–1094. (In Russ.)
  9. Certificate of state registration of computer programme “Electronic polyclinic” no. 2012614202. Date of registration: 12.05.2012. Mishlanov Ya.V., Mishlanov V.Yu., Mishlanova I.V., Mishlanova S.L. (In Russ.)
  10. Tishkov A.V., Khromov-Borisov N.N., Komashnya A.V., Marchenkova F.Yu., Semenova E.M., Eyubova Y.I., Delakova E.A., Bykhova A.V. The statistical analysis of tables 2.2 in diagnostic research. St. Petersburg, 2013, 17 p. (In Russ.)
  11. Federal clinical recommendations: “Chronic obstructive pulmonary disease”. Moscow, Russian Respiratory Society, 2018, 76 p. (In Russ.)
  12. Chuchalin A.G., Chereshnev V.A., Mishlanov V.Yu., Mishlanov Ya.V., Nikitin A.E., Shubin I.V. Bioethics, artificial intelligence and medical diagnostics. Perm, 2019, 208 р. (In Russ.)
  13. Bhatt S.P., Dransfield M.T. Chronic obstructive pulmonary disease and cardiovascular disease. Translational Research, 2013, vol. 162, no. 4, pp. 237–251.
  14. Sevenoaks M.J., Stockley R.A. Obstructive Pulmonary Disease, inflammation and co-morbidity – a common inflammatory phenotype? Respiratory Res., 2012, vol. 2, no. 7, p. 70.
  15. Sin D.D., Anthonisen N.R., Soriano J.B., Agusti F.G. Mortality in COPD: Role of comorbidities. European Respiratory Journal, 2006, vol. 28, no. 6, pp. 1245–1257.
KEYWORDS:

broncho-obstructive diseases, community-acquired pneumonia, chronic obstructive pulmonary disease, symptom, syndrome, interactive questionnaire.

FOR CORRESPONDENCE:

Change Implementation Centre at the Ministry of Health of Moscow Region

4, Karbyshev St., 143408, Krasnogorsk, Moscow Region, Russia

 Igor V. Shubin, Candidate of Medical Sciences, Deputy Director

Tel.: +7 (905) 500‑35‑30

Е-mail: shubin-igor@mail.ru

Perm State Medical University named after E.A. Wagner

26, Petropavlovskaya St., 614990, Perm, Russia

Vitaly Yu. Mishlanov, Doctor of Medical Sciences, Corresponding Member of the Russian Academy of Sciences, Head, Department of Internal Diseases Propedeutics No. 1

Tel.: +7 (950) 467‑76‑96

Е-mail: permmed@hotmail.com

Ekaterina P. Koshurnikova, Candidate of Medical Sciences, Docent, Department of Internal Diseases

Propedeutics No. 1

Tel.: +7 (912) 883‑22‑32

Е-mail: ekaterina_koshur@mail.ru

CLINICAL AND MICROBIOLOGICAL ISSUES OF THE DIAGNOSIS OF NONTUBERCULOUS MYCOBACTERIOSES IN PATIENTS WITH PULMONARY CAVITIES

Article 8.Page 73.
ARTICLE TITLE:

CLINICAL AND MICROBIOLOGICAL ISSUES OF THE DIAGNOSIS OF NONTUBERCULOUS MYCOBACTERIOSES IN PATIENTS WITH PULMONARY CAVITIES

DOI: 10.7868/S2587667820040081

AUTORS:

Karpina N.L., Asanov R.B., Shishkina E.R., Larionova E.E., Shabalina I.Yu., Ergeshov A.E.

Central TB Research Institute, Moscow, Russia

DESCRIPTION OF ARTICLE:

Submitted as of 19.07.2020

The incidence of pulmonary nontuberculous mycobacteriosis has significantly grown in all developed countries. We face severe challenges in the diagnosis of mycobacteriosis. In our research we analyzed examinations of 122 patients with pulmonary cavities and established mycobacterial infection in 82.8% of patients. The ratio between TB and mycobacteriosis was 41.8% and 41.2% respectively. We refuted the opinion that TB was most frequently diagnosed in patients with pulmonary cavities. The most common etiologic agent of mycobacteriosis in patients with pulmonary cavities was slowly growing mycobacteria, predominantly M. avium complex – 92% (46/50 patients). To exclude diagnostic mistakes in differential diagnosis of TB and pulmonary mycobacteriosis it is imperative to implement a special approach, including microbiological and molecular genetic verification of mycobacterial species. Absence of immunocompromised individuals among patients with verified pulmonary mycobacteriosis demonstrates that mycobacteriosis may also develop in individuals with intact immunity.

The study was conducted under research topic No. 0515-2019-0019 “A multidisciplinary approach to diagnosis, differential diagnosis of TB and other pulmonary diseases in the modern conditions”.

REFERENCES:
  1. Degtyareva S.A., Zaitseva A.S., Karpina N.L., Chernousova L.N., Stepanyan I.E., Shmelev E.I. Challenges in the treatment of an elderly patient with pulmonary tuberculosis and nontuberculous mycobacteriosis coinfection (A clinical case). Ru, 2017, vol. 139, no. 10, pp. 49–52. (In Russ.)
  2. Zimina V.N., Degtyareva S.Yu., Beloborodova E.N., Kulabukhova E.I., Rusakova L.I., Fesenko O.V. Mycobacterioses: the current state of the problem. Klinicheskaya mikrobiologiya i antimikrobnaya khimioterapiya, 2017, vol. 19, no. 4, pp. 276–282. (In Russ.)
  3. Lyamin A.V., Zhestkov A.V., Ismatullin D.D., Kovalev A.M. Laboratory diagnosis of mycobacterioses. The Bulletin of Contemporary Clinical Medicine, 2017, vol. 10, issue 1, pp. 29–35. (In Russ.) DOI: 10.20969/VSKM.2017.10(1).29–35
  4. Otten T.F., Vasilyev A.V. St. Petersburg, Meditsinskaya pressa, 2005, 224 p. (In Russ.)
  5. Daley C.L., Griffith D.E. Pulmonary non-tuberculous mycobacterial infections. J. Tuberc. Lung. Dis., 2010, vol. 14, no. 6, pp. 665–671.
  6. Haworth C.S., Banks J., Capstick T., Fisher A.J., Gorsuch T., Laurenson I.F., Leitch A., Loebinger M.R., Milburn H.J., Nightingale M., Ormerod P., Shingadia D., Smith D., Whitehead N., Wilson R., Floto R.A. British Thoracic Society guidelines for the management of non-tuberculous mycobacterial pulmonary disease (NTM – PD). Thorax, 2017, vol. 72 (Suppl 2), pp. ii1–ii64. DOI: 10.1136/thoraxjnl‑2017–210927
  7. Hoefsloot W., Van Ingen J., Andrejak C. et al. The geographic diversity of nontuberculous mycobacteria isolated from pulmonary samples: An NTMNET collaborative study. Respir. J., 2013, vol. 42, no. 6, pp. 1604–1613.
  8. Kai Ling Chin, Sarmiento M.E., Alvarez-Cabrera N., Norazmi M.N., Acosta A. Pulmonary non-tuberculous mycobacterial infections: current state and future management. J. Clin. Microbiol. Infect. Dis., 2020, vol. 39, no. 5, pp. 799–826. DOI: 10.1007/s10096‑019‑03771‑0.
  9. Koh W.J., Moon S.M., Kim S.Y., Woo M.A., Kim S., Jhun B.W., Park H.Y., Jeon K., Huh H.J., Ki C.S. et al. Outcomes of Mycobacterium avium complex lung disease based on clinical phenotype. Respir. J., 2017, vol. 50, no. 3, 1602503. DOI: 10.1183/13993003.02503-2016
  10. Prevots D.R., Loddenkemper R., Sotgiu G., Migliori G.B. Nontuberculous mycobacterial burden with substantial costs. Respir. J., 2017, vol. 49, no. 4, pii:1700374. DOI: 10.1183/13993003.00374-2017
  11. Stout J.E., Koh W.J., Yew W.W. Update on pulmonary disease due to nontuberculous mycobacteria. J. Infect. Dis., 2016, vol. 45, pp. 123–134.
  12. Ustinova V.V., Smirnova T.G., Sochivko D.G., Varlamov D.A., Larionova E.E., Andreevskaya S.N., Andrievskaya I.Yu., Kiseleva E.A., Chernousova L.N., Ergeshov A. New assay to diagnose and differentiate between Mycobacterium tuberculosis complex and nontuberculous mycobacteria. Tuberculosis, 2019, vol. 114, pp. 17–23. DOI: org/10.1016/j. tube.2018.10.004
  13. Van Ingen J., Boeree M.J., Dekhuijzen P.N., van Soolingen D. Environmental sources of rapid growing nontuberculous mycobacteria causing disease in humans. Microbiol. Infect., 2009, vol. 15, no. 10, pp. 888–893.
  14. Van Ingen J., Kohl T.A., Kranzer K., Hasse B., Keller P.M., Szafra K., Chand M., Schreiber P.W., Sommerstein R., Berger C., Genoni M., Rüegg C., Troillet N., Widmer A.F., Becker S.L., Hermann M., Eckmanns T., Haller S., Höller C., Debast S.B., Wolfhagen M.J., Hopman J., Kluytmans J., Langelaar M., Notermans D.W., Ten Oever J., Van den Barselaar P., Vonk A.B.A., Vos M.C., Ahmed N., Brown T., Crook D., Lamagni T., Phin N., Smith E.G., Zambon M., Serr A., Götting T., Ebner W., Thürmer A., Utpatel C., Spröer C., Bunk B., Nübel U., Bloemberg G.V., Böttger E.C., Niemann S., Wagner D., Sax H. Global outbreak of severe Mycobacterium chimaera disease after cardiac surgery: a molecular epidemiological study. Lancet Infect. Dis., 2017, vol. 17, no. 10, pp. 1033–1041. DOI: 10.1016/S1473–3099 (17) 30324‑9
  15. Wallace R.J. Jr., Iakhiaeva E., Williams M.D., Brown-Elliott B.A., Vasireddy S., Vasireddy R., Lande L., Peter son D.D., Sawicki J., Kwait R., Tichenor W.S., Turenne C., Falkinham J.O. 3rd. Absence of Mycobacterium intracellulare and presence of Mycobacterium chimaera in household water and biofilm samples of patients in the United States with Mycobacterium avium complex respiratory disease. J. Clin. Microbiol., 2013, vol. 51, no. 6, pp. 1747–1752.
  16. Won-Jung Koh. Nontuberculous Mycobacteria – Overview. Microbiol Spectr., 2017, vol. 5, no. 1, pp. 1–7. DOI: 10.1128/microbiolspec.TNMI7‑0024‑2016
  17. Yeonseok C. et al. Characteristics and outcomes of surgically resected solitary pulmonary nodules due to nontuberculous mycobacterial infections. J. Clin. Med., 2019, vol. 8, no. 11, pp. 1898. DOI: org/10.3390/jcm8111898
KEYWORDS:

nontuberculous mycobacteria, mycobacteriosis, cavities, diagnosis.

FOR CORRESPONDENCE:

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

Natalia L. Karpina, Doctor of Medical Sciences, Head of Centre for Diagnosis and Pulmonary

Rehabilitation, Deputy Director

Tel.: +7 (499) 785‑90‑26

Е-mail: natalya-karpina@rambler.ru

Ruslan B. Asanov, Laboratory Research Assistant, Centre for Diagnosis and Pulmonary Rehabilitation

Tel.: +7 (499) 785‑90‑26

Е-mail: doctor.asanov73@mail.ru

Ekaterina R. Shishkina, Junior Researcher, Centre for Diagnosis and Pulmonary Rehabilitation

Tel.: +7 (499) 785‑90‑26

Е-mail: ekaterina.eshishkina@yandex.ru

Elena E. Larionova, Candidate of Biological Sciences, Senior Researcher, Microbiology Department

Tel.: +7 (499) 785‑90‑91

Е-mail: larionova_lena@mail.ru 

Irina Yu. Shabalina, Candidate of Medical Sciences, Senior Researcher, Centre for Diagnosis and Pulmonary Rehabilitation

Tel.: +7 (499) 785‑90‑91

Е-mail: bronholog@yandex.ru

Atadzhan E. Ergeshov, Doctor of Medical Sciences, Professor, Director

Tel.: +7 (499) 785‑90‑19

Е-mail: cniit@ctri.ru

DIFFICULTIES IN DIFFERENTIAL DIAGNOSIS OF PULMONARY MYCOBACTERIOSIS CAUSED BY M. abscessus

Article 9.Page 81.
ARTICLE TITLE:

DIFFICULTIES IN DIFFERENTIAL DIAGNOSIS OF PULMONARY MYCOBACTERIOSIS CAUSED BY M. abscessus

DOI: 10.7868/S2587667820040093

AUTORS:

Sargsyan A.P., Makaryants N.N., Lepekha L.N., Bagirov M.A., Chernousova L.N., Kouklina G.M.

 Central TB Research Institute, Moscow, Russia

DESCRIPTION OF ARTICLE:

Submitted as of 06.10.2020

A clinical observation demonstrates difficulties in differential diagnosis of pulmonary mycobacteriosis in patients with misdiagnosed tuberculosis.

The article was prepared under research topic No. 0515-2019-0014 “Improvement of treatment methods for granulomatous, interstitial and nonspecific pulmonary diseases”.

REFERENCES:
  1. Guntupova L.D., Borisov S.E., Solovyeva I.P. et al. Mycobacterioses in phthisiopulmonology practice: a literature review and personal experience. Prakticheskaya meditsina, 2011, vol. 51, no. 3, pp. 39–50. (In Russ.)
  2. Zimina V.N., Degtyareva S.Yu., Beloborodova E.N., Kulabukhova E.I., Rusakova L.I., Fesenko O.V. Mycobacterioses: the current state of the problem. Klinicheskaya mikrobiologiya i antimikrobnaya khimioterapiya, 2017, vol. 19, no. 4, pp. 276–282. (In Russ.)
  3. Litvinov V.I. Nontuberculous mycobacteria, mycobacterioses. CTRI Bulletin, 2018, no. 2, pp. 5–20. (In Russ.)
  4. Makarova M.V., Guntupova L.D. Nontuberculous mycobacteria. Profilaktika, diagnostika, lecheniye, 2020, vol. 20, no. 2, pp. 97–102. (In Russ.)
  5. Pushkareva E.Yu., Alkhovik O.I. Diagnosis of pulmonary mycobacteriosis caused by abscessus (a clinical case). Tuberculosis and Lung Diseases, 2014, no. 1, pp. 47–50. (In Russ.)
  6. Solomai T.V. Epidemiological features of mycobacterioses caused by nontuberculous mycobacteria, Sanitarny vrach, 2015, no. 3, p. 30–36. (In Russ.)
  7. Dailloux M., Abalain M.L., Laurain C., Lebrun L., LoosAyav C., Lozniewski A., Maugein J., French Mycobacteria Study Group. Respiratory infections associated with nontuberculous mycobacteria in non-HIV patients. European Respiratory Journal, 2006, no. 28, 1211–1215.
  8. Griffith D., Aksamit T., Brown-Elliott B. et al. An official ATS/IDSA statement: diagnosis, treatment, and prevention of non-tuberculosis mycobacterial diseases. American Journal of Respiratory and Critical Care Medicine, 2007, vol. 175, issue 4, pp. 367–416.
  9. Heifets L. Mycobacterial infections caused by nontuberculous mycobacteria. Seminars in Respiratory and Critical Care Medicine, 2004, vol. 25, no. 3, pp. 283–295.
  10. Wassilew N., Hoffmann H., Andrejak C., Lange C. Pulmonary disease caused by non-tuberculous mycobacteria. Respiration, 2016, no. 91, pp. 386–402.
KEYWORDS:

mycobacteriosis, tuberculosis, rapidly growing nontuberculous mycobacteria.

FOR CORRESPONDENCE:

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

Anna P. Sargsyan, Postgraduate, Department of Differential Diagnosis of Pulmanory TB and Extracorporal Treatments

Tel.: +7 (499) 785‑91‑56

E-mail: a.sargsyan1993@mail.ru 

Natalia N. Makaryants, Doctor of Medical Sciences, Leading Researcher, Department of Differential Diagnosis of Pulmanory TB and Extracorporal Treatments, Head of Therapeutic Unit no. 2

Tel.: +7 (499) 785‑91‑56

E-mail: roman4000@yandex.ru

Larisa N. Lepekha, Doctor of Biological Sciences, Professor, Head, Department of Pathomorphology, Cell Biology and Biochemistry

Tel.: +7 (499) 785‑91‑79

Е-mail: lep3@yandex.ru

Mamed A. Bagirov, Doctor of Medical Sciences, Professor, Head, Surgery Department

Tel.: +7 (499) 785‑90‑60

Е-mail: bagirov60@gmail.com

Larisa N. Chernousova, Doctor of Biological Sciences, Professor, Head, Microbiology Department

Tel.: +7 (499) 785‑90‑91

Е-mail: lchernousova@mail.ru

Galina M. Kouklina, Candidate of Medical Sciences, Senior Researcher, Department of Differential Diagnosis of TB and Extracorporal Treatments

Tel.: +7 (499) 785‑91‑56

Е-mail: kgm74@mail.ru

DETECTION OF MYCOBACTERIA BY CULTURE INOCULATION. LIQUID MEDIA AND AUTOMATED SYSTEMS

Article 10.Page 88.
ARTICLE TITLE:

DETECTION OF MYCOBACTERIA BY CULTURE INOCULATION. LIQUID MEDIA AND AUTOMATED SYSTEMS

DOI: 10.7868/S258766782004010X

AUTORS:

Sevastyanova E.V., Smirnova T.G., Larionova E.E., Chernousova L.N.

Central TB Research Institute, Moscow, Russia

DESCRIPTION OF ARTICLE:

Submitted as of 08.09.2020

This article provides a brief review of methods of mycobacteria inoculation onto liquid nutrient media. A method of growing mycobacteria in the BACTEC MGIT 960 automated system is described.

The article was prepared under research topic No. 0515-2019-0015: “The development of drug resistance of mycobacteria and somatic cells to TB drugs”.

REFERENCES:
  1. Cultural methods of TB diagnosis. Educational manual for the basic training course for personnel from bacteriology laboratories of TB services. Ed. By V.V. Erokhin. Moscow-Tver, Triada, 2008, 208 p. (In Russ.)
  2. On improvement of TB control measures in the Russian Federation. Edict no. 109 by RF MoH as of 21.03.2003. Annex no. 11. Instruction for the unified methods of microbiological studies for detection, diagnosis and treatment of TB. (In Russ.)
  3. On endorsement of methodical recommendations on improvement of pulmonary TB diagnosis and treatment. Edict no. 951 by RF MoH as of 29.12.2014. (In Russ.)
  4. Sevastyanova E.V., Larionova E.E., Andrievskaya I.Yu., Smirnova T.G. Detection of mycobacteria by culture inoculation. Decontamination of diagnostic samples. CTRI Bulletin, 2020, no. 2, pp. 89–99. (In Russ.)
  5. Chernousova L.N., Puzanov V.A., Andreevskaya S.N., Smirnova T.G., Larionova E.E., Popov S.A. Laboratory diagnosis of TB. In: Methodical materials for thematic improvement cycle. Ed. by V.V. Erokhin, Moscow, 2012, 707 p. (In Russ.)
  6. Chernousova L.N., Sevastyanova E.V., Larionova E.E., Smirnova T.G., Andreevskaya S.N., Popov S.A., Zhuravlev V.Yu., Puzanov V.A., Maryandyshev A.O., Vakhrusheva D.V., Kravchenko M.A., Safonova S.G., Vasilyeva I.A., Ergeshov A.E. Federal clinical recommendations on organization and implementation of microbiological and molecular genetic diagnostics of TB. Moscow, RSPh, 2015, 35 p. (In Russ.)
  7. Mycobacteriology laboratory manual. Global Laboratory Initiative, 2014 Available at: http://www.stoptb.org/wg/gli/assets/documents/gli_mycobacteriology_lab_manual_web.pdf (Accessed 20 November 2018).
  8. Siddiqi S.H., Rüsch-Gerdes S. MGITTM Procedure Manual. For BACTEC™ MGIT 960™ TB System (Also applicable for Manual MGIT). Foundation for Innovative New Diagnostics, 2006, 89 p.
  9. Tortoli E., Cichero P., Piersimoni C., Simonetti T.M., Gesu G., Nista D. Use of BACTEC MGIT 960 for recovery of mycobacteria from clinical specimens: Multicenter study. Journal of Clinical Microbiology, 1999, vol. 37, no. 11, pp. 3578–3582.
KEYWORDS:

mycobacteria, liquid nutrient media, growth in the BACTEC MGIT 960 automated system.

FOR CORRESPONDENCE:

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

Elina V. Sevastyanova, Doctor of Biological Sciences, Leading Researcher, Microbiology Department

Tel.: +7 (499) 785‑90‑91

Е-mail: elinasev@yandex.ru

Tatiana G. Smirnova, Candidate of Medical Sciences, Senior Researcher, Microbiology Department

Tel.: +7 (499) 785‑90‑91

Е-mail: s_tatka@mail.ru

Elena E. Larionova, Candidate of Biological Sciences, Senior Researcher, Microbiology Department

Tel.: +7 (499) 785‑90‑91

E-mail: larionova_lena@mail.ru

Larisa N. Chernousova, Doctor of Biological Sciences, Professor, Head, Microbiology Department

Tel.: +7 (499) 785‑90‑91

Е-mail: lchernousova@mail.ru