Subscribe to “CTRI Bulletin”
Submit a paper

"CTRI Bulletin"
#3,(8),2019.

CTRI BULLETIN №3 (8) 2019

Journal Information: Read
Chief Editor: Ergeshov A.E.
Year of foundation: 2017
ISSN (Print): Browse
Publisher site: http://critub.ru
http://tb-bulletin.ru

Return to archive

CONTENTS

1)

Scientific achievements and perspectives of research of the Children and Adolescents’ tuberculosis Department, CTRI (dedicated to the 100th anniversary of Children and Adolescents’ Clinic, CTRI)

Ovsyankina E.S., Ergeshov A.E., Gubkina M.F.,Panova L.V., Yukhimenko N.V.

5 READ MORE
2)

The role of screening methods in TB detection in children

Gubkina M.F., Sterlikova S.S.

13 READ MORE
3)

Tuberculosis in children in the current context of the Saratov region: incidence, detection methods, clinical forms

Aleksandrova E.N., Morozova T.I.

20 READ MORE
4)

New approaches to chemotherapy of pulmonary MDR/XDR tuberculosis in children and adolescents after surgical treatment

Panova L.V., Ovsyankina. E.S., Khiteva A.Yu.

24 READ MORE
5)

The assessment of therapeutic сooperation between parents and health providers during TB treatment of children in hospital

Zolotova N.V., Petrakova I.Yu., Khokhlova J.Yu., Sterlikova S.S.

31 READ MORE
6)

Children with recurrent croup compose a high-risk group for TB

Salova A.L., Chelnokova O.G., Mozzhukhina L.I., Kogut T.A.

40 READ MORE
7)

Spontaneous healing of tuberculosis of intrathoracic lymph nodes in a young child

Petrakova I.Yu., Gubkina M.F., Sterlikova S.S, Gretsov E.M., Semenova L.A.

49 READ MORE
8)

Detection of fibro-cavernous pulmonary TB and treatment tactics

Punga V.V. , Yakimova M.A., Rusakova L.I., Sadovnikova S.S., Izmailova T.V.

56 READ MORE
9)

Immunological aspects of intolerance of antituberculosis drugs

Aberbakh M.M., Gergert V.Ya.

65 READ MORE
10)

Detection of mycobacteria by fluorescent microscopy. Part 1. Specimen preparation and staining

Sevastianova E.V., Larionova E.E., Andrievskaya I.Yu.

74 READ MORE

SCIENTIFIC ACHIEVEMENTS AND PERSPECTIVES OF RESEARCH OF THE CHILDREN AND ADOLESCENTS’ TUBERCULOSIS DEPARTMENT, CTRI (dedicated to the 100th anniversary of Children and Adolescents’ Clinic, CTRI)

Article 1.Page 5.
ARTICLE TITLE:

SCIENTIFIC ACHIEVEMENTS AND PERSPECTIVES OF RESEARCH OF THE CHILDREN AND ADOLESCENTS’ TUBERCULOSIS DEPARTMENT, CTRI (dedicated to the 100th anniversary of Children and Adolescents’ Clinic, CTRI)

DOI: 10.7868/S2587667819030014

AUTORS:

Ovsyankina E.S., Ergeshov A.E., Gubkina M.F., Panova L.V., Yukhimenko N.V.

Central TB Research Institute, Moscow, Russia

DESCRIPTION OF ARTICLE:

Submitted as of 19.04.2019

The article retrospectively presents the main directions of research activities of the Children and Adolescents’ TB Unit of the Federal State Budgetary Institution CTRI starting from 1919, as well as all heads of the clinic, the main directions and achievements of TB control activities in each time period and modern approaches in the anti-tuberculosis work.The article also covers the issues of diagnosis and differential diagnosis, specifics of tuberculosis (TB) treatment, psychological factors of TB etiopathogenesis and participation of the clinic staff in these scientific developments. It describes the goals set up for each of these areas and new technologies used to achieve them. All areas of research in the Children and Adolescents’ Clinic of the Research Institute are topical and promising in the field of children’s and adolescent’s phthisiology; they are of interest to researchers and practitioners.

REFERENCES:
  1. Gubkina, M.F., Khokhlova Yu.Yu., Yukhimenko N.V., Petrakova I.Yu. Personalized approaches to selection of respiratory tuberculosis chemotherapy regimens for children in contact with the index multidrug resistant TB cases. Tuberculosis and lung diseases, 2016, no. 9, pp. 24–30. (In Russ.)
  2. Zolotova N.N., Akhtyamova A.A., Streltsov V.V., Baranova G.V., Ovsyankina E.S., Yukhimenko N.V. Individual characteristics of children and adolescents with respiratory tuberculosis in the presence or absence of contact with the source of infection. Tuberculosis and lung disease, 2017, Vol. 95, no. 3, pp. 41–47. (In Russ.)
  3. Markuzon V.D. Management and specifics of the children’s sanatorium. Childhood tuberculosis, 1923, 1, pp. 4–20. (In Russ.)
  4. Guidelines for the diagnosis of less evident forms of tuberculosis of the intrathoracic lymph nodes, M., 1983, p. 11. (In Russ.)
  5. Mitinskaya L.A. Antituberculosis BCG revaccination. (Library of a clinician), M.: Medicine, 1975, p. 152. (In Russ.)
  6. Mitinskaya L.A. Tuberculosis in children, M.: JSC Kudesniki, 2004, p. 196. (In Russ.)
  7. Ovsyankina E.S., Gubkinа M.F., Panova L.V., Kobulashvili M.G. Chemotherapy of tuberculosis in children and adolescents and proposed solutions. Problems of tuberculosis and lung disease, 2006, no. 8, pp. 26–29. (In Russ.)
  8. Ovsyankina E.S., Gubkina M.F., Panova L.V. Methods of TB infection screening in children, Russian pediatric journal, 2017, Vol. 2, pp. 108–115. (In Russ.)
  9. Panova L.V., Ovsyankina E.S., Sadovnikova S.S., Khiteva A.Y., Chemotherapy of the respiratory system tuberculosis in older children and adolescents after surgical treatment. Tuberculosis and lung disease, 2017, 95, no. 5, pp. 41–45. (In Russ.)
  10. Pokhitonova M.P. Clinical manifestations, therapy and prevention of tuberculosis in children, M., Medgiz, 1952, p. 303. (In Russ.)
  11. Firsova V.A. Specifics of the course of tuberculosis in adolescents. Tuberculosis of the respiratory system, Manual for physicians ed. by A.G. Khomenko, M., Medgiz, 1988, pp. 293–298. (In Russ.)
  12. Firsova V.A. Tuberculosis in adolescents, M., PTP Printing house Science, 2010, p. 223.
  13. Chemoprophylaxis of tuberculosis (guideline), M., 1988, p. 10. (In Russ.)
  14. Yukhimenko N.V. Revaccination of Schoolchildren with BCG-M Vaccine and the Use of a new model of needle-free injector BI‑19. Problems of tuberculosis, 1992, no. 2, pp. 18–20. (In Russ.)
  15. Ergeshov A.E. Liver condition ultrasound monitoring in children with thoracic tuberculosis during chemotherapy. Problems of tuberculosis, 1989, no. 9, pp. 48–51. (In Russ.)
  16. WHO consolidated guidelines on drug-resistant tuberculosis treatment 2019. – 104 р. www.who.int/tb/publications/2019/consolidated-guidelines-drug-resistant-TB-treatment/en/
KEYWORDS:

tuberculosis, children, adolescents, prevention, detection, diagnosis, treatment, scientific achievements, new technologies.

FOR CORRESPONDENCE:

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

Elena S. Ovsyankina, Doctor of Medical Science, professor, Head of Children and Adolescents’

TB Department

Tel.: +7 (499) 785-90-05

E-mail: detstvocniit@mail.ru

Atadzhan E. Ergeshov, Doctor of Medical Science, Professor, Director

Tel: +7 (499) 785-90-19

E-mail: cniit@ctri.ru

Marina F. Gubkinа, Doctor of Medical Science, Principal Research Scientist, Children and Adolescents’

TB Department

Tel: +7 (499) 785-90-05

E-mail: detstvocniit@mail.ru

Lyudmila V. Panova, Doctor of Medical Science, Leading Research Scientist, Children and Adolescents’

TB Department

Tel.: +7 (499) 785-90-05

E-mail: detstvocniit@mail.ru

Natalia V. Yukhimenko, Doctor of Medical Science, Leading Research Scientist, Children and Adolescents’ TB Department

Tel.: +7 (499) 785-90-05

E-mail: detstvocniit@mail.ru

THE ROLE OF SCREENING METHODS IN TB DETECTION IN CHILDREN

Article 2.Page 13.
ARTICLE TITLE:

THE ROLE OF SCREENING METHODS IN TB DETECTION IN CHILDREN

DOI: 10.7868/S2587667819030026

AUTORS:

Gubkina M.F.1, 2, Sterlikova S.S.1

1Central TB Research Institute, Moscow, Russia

2Russian National Research Medical University named after N.I. Pirogov, Moscow, Russia

DESCRIPTION OF ARTICLE:

Submitted as of 04.06.2019

Aim: to assess the role of mass or individual screening for TB in children from known or unknown TB infection sources.

Materials and methods. We studied 72 children (aged 3–12) with active pulmonary TB in 2012–2017. We divided them in two groups: group 1 – 43 children, who had not been followed up during contact tracing before TB detection; group 2 – 29 children, who had been followed up during contact tracing before TB detection.

Results. We found defects in organization of mass skin testing: in 43.1% of cases skin testing with 2 TU PPD-L was performed less than once a year; conversion of skin reactions was not reported in 71.9% of cases. We also found defects in dispensary follow-up of group 2: first, 17.2% of cases were studied twice a year during the 1st year of contact tracing, other cases (82.8%) – once a year at least; second, 86.2% of cases underwent testing with recombinant tuberculosis allergen (RTA) at registration, 85.7% – after one year of follow-up, 71.4% – after 2 years of follow-up. In group 2, TB was detected in 51.7% of cases after one year, 10.3% – after 2 years, 38.0% – later; in all these cases TB was detected by chest CT. The terms and indications for chest CT were determined randomly in most cases (80.0%). The symptoms in cases detected by chest CT were either absent or slight. Screening detection of TB prevailed in the both groups: group 1 – 74.4% (37.2% by simultaneous study of contacts and the index case; 37.2% by mass skin testing), group 2 – 89.7% (75.9% during contact tracing, 13.8% by mass skin testing). At the same time, in 25.6% cases of group 1 and 10.3% cases of group 2 TB was detected at referrals to medical institutions and had acute onset. There were no differences between the groups by detection methods (χ2 = 5,684, р > 0.05).

Conclusion. The main causes of late TB detection in children were improper organization of screening or dispensary followup. The algorithm of studies (indications, terms) including chest CT for children from TB risk group should be expanded and focused on both positive reactions to RTA and dynamic changes of skin testing results, consider a family social status, a clinical form and course of TB in the index case.

The research was performed under scientific theme № 0515-2019-0016 “Personalized approaches to the treatment of respiratory TB in children and adolescents”.

REFERENCES:
  1. Aksenova V.A., Klevno N.I., Kavtarashvili S.M. The TB infection source and its role in TB development in children. Tuberculosis and Lung Diseases, 2015, no. 1, pp. 19–24. (In Russ.)
  2. Manual for the application of DIASKINTEST. Recombinant tuberculosis allergen in the standard dilution, solution for intradermal administration. Ratified on 19.06.2008, No. 01-11/99-08. (In Russ.)
  3. Kotova L.I., Mitinskaya L.A., Yandarova V.V. Tuberculinodiagnosis as a method of TB detection in children from high risk groups. Proceedings of the 8th All-Union Congress of Phthisiologists. 1976, pp. 262–265. (In Russ.)
  4. Lazareva Ya.V. Lungs and mediastinum computed tomography of children from high risk groups for Problems of Tuberculosis, 2001, no. 1, pp. 17–19. (In Russ.)
  5. Mitinskaya L.A., Kotova L.I., Yandarova V.V. Research into potential applications of a single tuberculin test in mass screenings of children and adolescents for TB. Problems of Tuberculosis, 1970, no. 10, pp. 1–4. (In Russ.)
  6. On amendments to Annex 4 to the Russian MoH Order no. 109 of 21.03.2003. Order no. 855 of 29.10.2009 by the Ministry of Health and Social Development of the Russian Federation. (In Russ.)
  7. Ovsyankina E.S., Gubkina M.F. The main risk factors for TB development in children and adolescents. Problems of Tuberculosis, 2005, no. 1, pp. 10–13. (In Russ.)
  8. Sanitary and epidemiological rules, SP 3.1.2.3114-13: Prevention of TB, no 60 of 22.10.2013. (In Russ.)
  9. Tyurin I.E. The diagnostic algorithm after detection of focal changes in the lungs by high-resolution computed tomography. Vestnik rentgenologii i radiologii, 2013, no. 6, pp. 44–50. (In Russ.)
  10. Federal clinical recommendations on diagnosis and treatment of latent TB infection in children. Moscow, ROOI “Zdorovie cheloveka”, 2015, 36 p. (In Russ.)
  11. Shepeleva L.P., Aksenova V.A., Tyurin I.E. The characteristics of lung foci detected in children and adolescents from TB risk groups. Vestnik rentgenologii i radiologii, 2014, no. 1, pp. 5–9. (In Russ.)
  12. Shovkun L.A., Ilyina O.V. The TB infection source: the current state, problems. Tuberculosis and Lung Diseases, 2014, no. 8, pp. 116–118. (In Russ.)
KEYWORDS:

TB detection, children, infection source, skin immunological tests, screening.

FOR CORRESPONDENCE:

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

Russian National Research Medical University named after N.I. Pirogov 1, Ostrovityanova St., 117997, Moscow, Russia

Marina F. Gubkina, Doctor of Medical Sciences, Principal Researcher, Children and Adolescents’ Department, Central TB Research Institute; Professor, Phthisiology Department, Russian National Research Medical University named after N.I. Pirogov

Tel.: +7 (499) 785-90-27

E-mail: detstvocniit@mail.ru

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

Svetlana S. Sterlikova, Phthysiologist, Junior Division, Children and Adolescents’ Department

Tel.: +7 (499) 785-90-27

E-mail: detstvocniit@mail.ru

TUBERCULOSIS IN CHILDREN IN THE CURRENT CONTEXT OF THE SARATOV REGION: INCIDENCE, DETECTION METHODS, CLINICAL FORMS

Article 3.Page 20.
ARTICLE TITLE:

TUBERCULOSIS IN CHILDREN IN THE CURRENT CONTEXT OF THE SARATOV REGION: INCIDENCE, DETECTION METHODS, CLINICAL FORMS

DOI: 10.7868/S2587667819030038

AUTORS:

Aleksandrova E.N., Morozova T.I.

Saratov State Medical University n.a. V.I. Razumovsky, Russian Ministry of Health, Saratov, Russia

DESCRIPTION OF ARTICLE:

Submitted as of 14.06.2019

Improvement in childhood TB incidence is closely related to the major early TB detection efforts confirmed by active

TB detection in 84.4% of cases, including with the use of tuberculin diagnosis and Diaskintest. The TB diagnostics was performed in 88.5% of patients under observation in the follow-up diagnostic group “0”.In every second child (54.1%), the diagnosis of active tuberculosis was confirmed by the chest computer tomography examination. Among clinical forms, TB of the intrathoracic lymph nodes was most prevalent; TB forms with complications were diagnosed in 15.8% of cases. Every third child with tuberculosis had a history of contact with a TB patient. At the same time, the index cases were diagnosed with multidrug and extensively drug resistant M. tuberculosis in 73.7% of cases.

REFERENCES:
  1. Vasilieva I.A., Belilovsky E.M., Borisov S.E., Sterlikov S.A. Incidence, mortality and prevalence as burden indicators in the WHO regions, countries of the world and in the Russian Federation, Part 1, Incidence and prevalence of tuberculosis, Tuberculosis and lung disease, 2017, vol. 95, no. 6, p. 9–21. (In Russ.)
  2. Executive Order of the Russian Ministry of Health no. 951 of 29.12.2014 “On Approval of Guidelines for Improving Diagnosis and Treatment of Respiratory Tuberculosis”. (In Russ.)
KEYWORDS:

children, tuberculosis incidence, detection methods, clinical forms, tuberculosis foci.

FOR CORRESPONDENCE:

FSBEI Saratov State Medical University n.a. V.I. Razumovsky, Ministry of Health, Russian Federation

112, Bolshaya Kazachiya str., 410012, Saratov

Elena N. Alexandrova, Assistant Professor, Phthisiology Department, Candidate of Medical Science

Tel: +7 (917) 306-66-67

E-mail: allenik238@mail.ru

Tatyana I. Morozova, Head of Phthisiology Department, Doctor of Medical Science, Professor

Tel: +7 (8452) 26-16-90

E-mail: kafedra_ftiz@inbox.ru

NEW APPROACHES TO CHEMOTHERAPY OF PULMONARY MDR/XDR TUBERCULOSIS IN CHILDREN AND ADOLESCENTS AFTER SURGICAL TREATMENT

Article 4.Page 24.
ARTICLE TITLE:

NEW APPROACHES TO CHEMOTHERAPY OF PULMONARY MDR/XDR TUBERCULOSIS IN CHILDREN AND ADOLESCENTS AFTER SURGICAL TREATMENT

DOI: 10.7868/S258766781903004X

AUTORS:

Panova L.V., Ovsyankina. E.S., Khiteva A.Yu.

Central TB Research Institute, Moscow, Russia

DESCRIPTION OF ARTICLE:

Submitted as of 17.06.2019

The cohort prospective study included 50 patients aged 13–17 under surgical treatment of respiratory tuberculosis (TB) provided at the CTRI between 2015–2017. Drug susceptibility data at the admission were obtained in 35 of 50 cases, of which 18 (51.4%) had multidrug/extensively drug resistant (MDR/XDR) tuberculosis. Based on the results of the microbiological examinations of surgical specimens, MDR was newly detected in 3 cases. Criteria developed for selection of proper duration of chemotherapy (CT) can be used in patients with MDR/XDR M. tuberculosis. Of 21 patients after surgical treatment, 3 received chemotherapy for 3 months, 8 – for 6 months, and 9 – for more than 6 months. In one case, chemotherapy was not performed due to the development of pancreatitis. Both the  ffectiveness and safety of the designed regimens were proved by the absence of relapses a year after the completion of the main course of chemotherapy.

The research was performed under scientific theme № 0515-2019-0016 “Personalized approaches to the treatment of respiratory TB in children and adolescents”.

REFERENCES:
  1. Clinical recommendations Respiratory Tuberculosis in Children, RSP, 2006, p. 45 http://roftb.ru/netcat_files/doks2017/kl_3.pdf (In Russ.)
  2. Key TB indicators in the Russian Federation in 2017, Federal Center for Monitoring Counteractions Against Tuberculosis Spread in the Russian Federation, Central Research Institute of Health Care Organization and Informatization, Russian Ministry of Health, http://mednet.ru/images/stories/tb2017.pdf (In Russ.)
  3. Panova L.V., Ovsyankina E.S., Giller D.B., Kobulashvili M.G. Algorithms for Treatment of Destructive Pulmonary Tuberculosis in Older Children and Adolescents (newly detected and retreated) // Tuberculosis and Socially Significant Diseases, 2014, no. 1–2, p. 125–126. (In Russ.)
  4. WHO consolidated guidelines on drug-resistant tuberculosis treatment. 2019. – 104 р. www.who.int/tb/publications/2019/consolidated-guidelinesdrug-resistant-TB-treatment/en/
KEYWORDS:

children, adolescents, multiple/extensively drug resistance of Mycobacterium tuberculosis, TB chemotherapy.

FOR CORRESPONDENCE:

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

Lyudmila V. Panova, Doctor of Medical Science, Leading Researcher, Children and Adolescents’

TB Department

Tel.: +7 (499) 785-90-05

E-mail: detstvocniit@mail.ru

Elena S. Ovsyankina, Doctor of Medical Science, Professor, Head of Children and Adolescents’

TB Department

Tel.: +7 (499) 785-90-05

E-mail: detstvocniit@mail.ru

Antonina Yu. Khiteva, Junior Research Scientists, Children and Adolescents’ TB Department

Tel.: +7 (499) 785-90-05

E-mail: detstvocniit@mail.ru

THE ASSESSMENT OF THERAPEUTIC COOPERATION BETWEEN PARENTS AND HEALTH PROVIDERS DURING TB TREATMENT OF CHILDREN IN HOSPITAL

Article 5.Page 31.
ARTICLE TITLE:

THE ASSESSMENT OF THERAPEUTIC COOPERATION BETWEEN PARENTS AND HEALTH PROVIDERS DURING TB TREATMENT OF CHILDREN IN HOSPITAL

DOI: 10.7868/S2587667819030051

AUTORS:

Zolotova N.V., Petrakova I.Yu., Khokhlova J.Yu., Sterlikova S.S.

Central TB Research Institute, Moscow, Russia

DESCRIPTION OF ARTICLE:

Submitted as of 01.10.2018

The aim of the research was to investigate the peculiarities of therapeutic cooperation between parents and health providers during inpatient treatment of children with pulmonary TB.

Materials and methods. The research enrolled 114 parents of children aged 3–12. The children had been admitted to hospital with diagnosed pulmonary TB and received inpatient treatment for 2–18 months. To assess the cooperation between parents and health providers, we developed a questionnaire, which outlined the main parameters of parents’ attitude to the treatment situation and the peculiarities of cooperation with health providers. The attending physician performed ranged quantitative assessment at the initial stage of chemotherapy and after 2–4 months of hospital treatment. Based on the complex analysis of the obtained data, we determined three groups of parents with low, satisfactory or high level of therapeutic cooperation.

Results. We established that parents with high or satisfactory level of cooperation reliably prevailed (40.4% in each group) versus parents with low level of compliance (19.2%) (р < 0.05). We also established that high compliance was associated with stable parameters of therapeutic cooperation (81.4%). The level of therapeutic cooperation was determined by the parent’s attitude to the child’s treatment in hospital. We described the peculiarities of therapeutic cooperation with parents, who had different attitudes to hospital treatment.

Conclusion. The therapeutic cooperation between parents and health providers was determined by the family social status and the circumstances of the child’s treatment (referrals from other Russian regions or former Soviet republics).

The research was performed under scientific theme № 0515-2015-0001 “Psychological aspects of pulmonary TB treatment in children from TB infection sources or social risk groups”.

REFERENCES:
  1. Aksenova V.A., Klevno N.I., Kavtarashvili S.M., Kazakov A.V., Pakhlavonova A.D. A TB infection source as a risk factor for multi-drug resistant TB in children. Tuberculosis and Lung Diseases, 2018, no. 1, pp. 11–16. (In Russ.)
  2. Danikov D.S. Therapeutic cooperation (compliance): a concept, mechanisms of development and methods of optimization. Nevrologia, neiropsikhiatriya, psikhosomatika, 2014, no. 2, pp. 4–12. (In Russ.)
  3. Kaplunov K.O. Optimization of physician-child’s relatives’ relationship in clinical childhood infections. Thesis for a Candidate’s degree in specialty no. 14.02.05: Sociology of medicine. Volgograd, 2014, 146 p. (In Russ.)
  4. Mikhailova S.V., Krivokhizh V.N. The influence of social risk factors on TB infection in children from household TB sources. Tuberculosis and Lung Diseases, 2015, no. 7, pp. 89–90. (In Russ.)
  5. Motanova L.N., Russkikh N.Yu. The peculiarities of TB course in children and adolescents from socially disadvantaged families. Tuberculosis and Lung Diseases, 2011, no. 1, pp. 16–21. (In Russ.)
  6. Ovsyankina E.S., Panova L.V., Poluetkova F.A., Khiteva A.Yu., Viechelli E.A. Urgent issues of TB in adolescents from TB infection sources. Tuberculosis and Lung Diseases, 2018, no. 6, pp. 17–20. (In Russ.)
  7.  Rashkevich E.E., Myakisheva T.V., Avdeeva T.G. Social risk factors and their influence on nature and course of TB in adolescents. Sibirskoe medicinskoe obozrenie, 2011, no. 6, pp. 97–99. (In Russ.)
KEYWORDS:

pulmonary TB, children, parents, therapeutic relationship, attitude to treatment.

FOR CORRESPONDENCE:

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

Natalia V. Zolotova, Candidate of Psychological Sciences, Senior Researcher, Psychology Team, Children and Adolescents’ Department

Tel.: +7 (499) 785-90-27

Е-mail: Zolotova_n@mail.ru

Irina Yu. Petrakova, Candidate of Medical Sciences, Head, Children’s Division

Tel.: +7 (499) 785-90-27

Е-mail: detstvocniit@mail.ru

Julia Yu. Khokhlova, TB physician, Children’s Division

Tel.: +7 (499) 785-90-27

Е-mail: detstvocniit@mail.ru

Svetlana S. Sterlikova, TB physician, Children’s Division

Tel.: +7 (499) 785-90-27

Е-mail: detstvocniit@mail.ru

CHILDREN WITH RECURRENT CROUP COMPOSE A HIGH-RISK GROUP FOR TB

Article 6.Page 40.
ARTICLE TITLE:

CHILDREN WITH RECURRENT CROUP COMPOSE A HIGH-RISK GROUP FOR TB

DOI: 10.7868/S2587667819030063

AUTORS:

Salova A.L., Chelnokova O.G., Mozzhukhina L.I., Kogut T.A.

Yaroslavl State Medical University, Yaroslavl, Russia

DESCRIPTION OF ARTICLE:

Submitted as of 03.02.2019

We studied the relationship between TB infection and croup syndrome in children. The study of 304 children revealed

that in 94–96% of cases the first croup episode coincided with the early period of M. tuberculosis infection. TB incidence among children with recurrent croup reached 27%. The termination of croup episodes under TB treatment was observed in 82% of cases, which confirmed the influence of TB infection on early or recurrent course of croup in children. The study established that children with croup syndrome compose a high-risk group for TB. TB infection, as well as active TB, contribute to recurrent course of croup.

REFERENCES:
  1. Aksenova V.A., Sevostyanova T.A. TB in children and adolescents in Russia. Lechashchy vrach, 2013,
  2. 1, pp. 35–39. (In Russ.)
  3. Artyukhina L.Yu., Ivanova E.S., Frolova N.F., Kotenko O.N., Shirinsky V.G., Khokhlatov D.E., Varyasin V.V., Tomiliga N.A. The peculiarities of TB infection course under immunosuppression in kidney recipients. Lechebnoe delo, 2015, no. 1, p. 11. (In Russ.)
  4. Kogut T.A., Mozzhukhina L.I., Ganicheva N.P., Emelicheva L.G. The modern recommendations on management of stenosing laryngotracheitis in children. Izvestia Samarskogo nauchnogo centra Rossiiskoi akademii nauk, 2014, vol. 15, no. 5, pp. 718–719. (In Russ.)
  5. Ovsyankina E.S., Gubkina M.F. The principles of TB diagnosis in children at referrals to medical institutions. Consilium Medicum. Pediatria (Annex), 2008, no. 1, pp. 85–87. (In Russ.)
  6. Polyakova A.S., Bakradze M.D., Tatochenko V.K. Croup syndrome in children: prejudice and evidence-based medicine. Farmateka, 2018, no. 1, pp. 17–18. (In Russ.)
  7. Pokhitonova M.P. Clinical TB, management and prevention in children. Moscow, Medicine, 1965, 32–54, 84–154. (In Russ.)
  8. Firsova V.A. Pulmonary TB in children. Moscow, Medicine, 1978. (In Russ.)
  9. Yarygin N.E. Pathomorphology of vegetative nervous system in TB. Moscow, Medgiz, 1906, pp. 5–32, 173–212. (In Russ.)
  10. Abedi G.R., Prill M.M., Langley G.E., Wikswo M.E., Weinberg G.A., Curns A.T., Schneider E. Estimates of parainfluenza virus-associated hospitalizations and cost among children aged less than 5 years in the United States, 1998–2010. J. Pediatric Infect. Dis Soc., 2016, vol. 5, no. 1, pp. 7–13.
  11. Johnson D.W. Croup. BMJ Clin. Evid., 2014, vol. 2014, pp. 321.
  12. Worrall G. Croup. Can. Fam. Physician, 2008, vol. 54, no. 4, pp. 573–574.
KEYWORDS:

croup syndrome, TB, paraspecific reaction, M. tuberculosis.

FOR CORRESPONDENCE:

Yaroslavl State Medical University

5, Revolyutsionnaya St., 150000, Yaroslavl, Russia

Aleksandra L. Salova, Assistant, Phthisiology Department

Tel.: +7 (996) 697-01-54

E-mail: domalexandra@mail.ru

Olga G. Chelnokova, Doctor of Medical Sciences, Docent, Head of Phthisiology Department

Tel.: +7 (915) 976-93-73

E-mail: chelnokova@bk.ru

Lidiya I. Mozzhukhina, Doctor of Medical Sciences, Professor, Head of Pediatric Department, Institute of Postgraduate and Additional Education

Tel.: +7 (961) 155-13-14

E-mail: mli1612@mail.ru

Tatyana A. Kogut, Candidate of Medical Sciences, Docent, Pediatric Department,

Institute of Postgraduate and Additional Education

Tel.: +7 (903) 824-93-54

E-mail: tkogut@bk.ru

SPONTANEOUS HEALING OF TUBERCULOSIS OF INTRATHORACIC LYMPH NODES IN A YOUNG CHILD

Article 7.Page 49.
ARTICLE TITLE:

SPONTANEOUS HEALING OF TUBERCULOSIS OF INTRATHORACIC LYMPH NODES IN A YOUNG CHILD

 DOI: 10.7868/S2587667819030075

AUTORS:

Petrakova I.Yu.1, Gubkina M.F.1,2, Sterlikova S.S.1, Gretsov E.M.1, Semenova L.A.1

1FSBI Central TB Research Institute, Moscow, Russia

2FSEI Russian National Research Medical University. n. a. N.I. Pirogov, Russian Ministry of Health, Moscow, Russia

DESCRIPTION OF ARTICLE:

Submitted as of 17.06.2019

This is a presentation of the clinical observation showing asymptomatic course of tuberculosis (TB) in a child vaccinated against TB in the presence of multiple TB risk factors (social, medical, epidemic).

The purpose of the demonstration: to show the effectiveness of TB immunization that prevented generalization of TB in a child who was in contact with a TB patient – his father – from the very first months of his life, and contributed

to the asymptomatic course of tuberculosis and spontaneous cure of TB.

The research was performed under scientific theme № 0515-2019-0016 “Personalized approaches to the treatment of respiratory TB in children and adolescents”.

REFERENCES:
  1. Aksenova V.A. Tuberculosis in children and adolescents, Textbook, 2007, p. 230. (In Russ.)
  2. Zemskova Z.S., Dorozhkova I.R., Asymptomatic tuberculosis infection, M., 1984. p. 112–177. (In Russ.)
  3. Koretskaya N.M., Modern insights in BCG vaccination, Siberian medical review, 2011, no. 1, p. 3–8. (In Russ.)
  4. Maslova O.V., Kiselevich O.K.,, Vlasova E.E., Yusupova A.N., Bogdanova E.V. Primary tuberculosis in young children with the household TB contacts, Tuberculosis and lung disease, 2014, no. 8, p. 67–68. (In Russ.)
  5. Mitinskaya, L.A. 80 years of BCG vaccine application, Problems of tuberculosis, 2001, no. 1, p. 51–53. (In Russ.)
  6. Mitinskaya L.A. BCG Vaccination (present and future), Problems of tuberculosis, 1995, no. 3, p. 54–58. (In Russ.)
  7. Poddubnaya L.V. BCG Vaccination and its effectiveness in children, Problems of tuberculosis, 2006, 1, p. 13–16. (In Russ.)
  8. Executive Order of March 21, 2014 no. 125n “On approval of the national calendar of preventive vaccinations and the calendar of preventive vaccinations based on the epidemiological indications”. (In Russ.)
  9. Sanitary and epidemiological rules SR3.1.2.3114-13 “Prevention of tuberculosis”, no. 60, ed. on 06.02.2015. (In Russ.)
  10. Starshinova A.A., Pavlova M.V., Dovgalyuk I.F, Ovchinnikova Yu.E., Yalfimov A.N. Influence of risk factors on the development and course of tuberculosis infection in children with the household TB contacts in the current socio-epidemic situation, Tuberculosis and lung disease, 2010, no. 6, p. 34–39. (In Russ.)
  11. Phthisiology: national guidelines, ed. by M.I. Perelman, M.: GEOTAR-Media, 2007, p. 512. (In Russ.)
  12. BCG vaccines: WHO position paper – February 2018. Weekly epidemiological record, 2018, 8(93), 73–96.
  13. Kaufmann E., Sanz J., Dunn J.L., Khan N., Mendonс‚ a L.E., Pacis A. et al. BCG educates hematopoietic stem cells to generate protective innate immunity against tuberculosis. Cell. 2018, 172: e119.
  14. Levy O., Wynn J.L. A prime time for trained immunity: innate immune memory in newborns and infants. Neonatology, 2014, 105:136–41.
KEYWORDS:

tuberculosis, young children, focus of tuberculosis infection, vaccination against tuberculosis.

FOR CORRESPONDENCE:

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

Irina Yu. Petrakova, Candidate of Medical Sciences, Head, Children’s Division

Tel: +7 (499) 785-90-27

Email: detstvocniit@mail.ru

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

Russian National Research Medical University. n. a. N.I. Pirogov, Russian Ministry of Health, Moscow, Russia

1, Ostrovityanova Street, 117997, Moscow, Russia

Marina F. Gubkina, Doctor of Medical Sciences, Principal Researcher, Professor, Phthisiology Department

Tel: +7 (499) 785-90-27

E-mail: detstvocniit@mail.ru

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

Svetlana S. Sterlikova, physician, Children’s Division

Tel: +7 (499) 785-90-27

E-mail: detstvocniit@mail.ru

Evgeny M. Gretsov, pathologist, Department of Pathology

Tel: + 7 (926) 254-85-15

E-mail: gem2505@yandex.ru

Lyudmila A. Semenova, Candidate of Medical Science, Senior Researcher, Department of Pathology,

Cell Biology and Biochemistry

Tel: + 7 (905) 794-98-22

E-mail: lu.kk@yandex.ru

DETECTION OF FIBRO-CAVERNOUS PULMONARY TB AND TREATMENT TACTICS

Article 8.Page 56.
ARTICLE TITLE:

DETECTION OF FIBRO-CAVERNOUS PULMONARY TB AND TREATMENT TACTICS

DOI: 10.7868/S2587667819030087

AUTORS:

Punga V.V., Yakimova M.A., Rusakova L.I., Sadovnikova S.S., Izmailova T.V.

Central TB Research Institute, Moscow, Russia

DESCRIPTION OF ARTICLE:

Submitted as of 01.02.2019

Aim: To study the epidemiological situation and the factors contributing to development of fibro-cavernous pulmonary TB (FCPT); characterize patients, who received surgical treatment at the Central TB Research Institute (CTRI).

Materials and methods. We analyzed the data from a single statistical observation, studied reporting forms no. 33 and no. 8 containing the information about the number of notified TB cases with cavities and FCPT obtained from several Russian regions in 2014–2017. We performed a retrospective analysis of 436 case records of FCPT patients admitted to CTRI in 2014–2017 for surgery.

Results. Late detection of FCPT was conditioned by quality of preventive screenings, diagnostic methods applied in the Russian regions, performance of primary medical institutions. The prevalence of FCPT significantly varied in different regions due to local specific conditions and differences in organization of TB services. Effectiveness of FCPT treatment was not high due to: a large proportion of treatment interruption cases; a large proportion of multi-drug resistant cases; non-adherence to treatment standards; and poor organization of directly-observed treatment. Surgery for FCPT tends to grow; recently the volume of surgical interventions has twice increased but remains insufficient. Application of surgical methods increases treatment effectiveness to 91.5%.

The study was performed under research theme № 0515-2019-0015 “Modern approaches to diagnosis, epidemiology and management of drug resistant pulmonary TB, including HIV-associated and diabetes mellitus-associated TB”.

REFERENCES:
  1. Vasilyeva I.A., Belilovsky E.M., Borisov S.E., Sterlikov S.A. WHO global tuberculosis reports: compilation and interpretation. Tuberculosis and Lung Diseases, 2017, no. 5, pp. 1–14. (In Russ.)
  2. Bagirov M.A., Sadovnikova S.S., Krasnikova E.V., Albekov R.Z., Aliev V.K., Prokhodtsev D.N., Ergeshov A.E. The present roles of collapse surgery in the complex treatment of advanced pulmonary TB with multidrug resistance. In: Scientific research and the institute’s history essays. Ed. by A.E. Ergeshov, Moscow, Galleya-Print, 2016, pp. 83–95. (In Russ.)
  3. Mukhamedov Kh.Kh., Sabirov Sh.Yu., Kayumova S.S., Khasanov Kh.Z. The ways to improve effectiveness of lung resection in patients with destructive or complicated drug resistant pulmonary TB. Molodoi ucheny, 2016, no. 4, pp. 287–289. (In Russ.)
  4. The main indicators of TB in the Russian Federation in 2016. Electronic resource, Central Research Institute of Organization and Informatization of Health [official site]. (In Russ.) URL: http//www.mednet.ru/ru czentr-monitoringa-tuberkuloz html (15/03/2017).
  5. Sterlikov S.A., Nechaeva O.B., Kucheryavaya D.A., Obukhova O.V., Chebagina T.Yu. Sectoral and economic indicators of TB control in 2015–2016. Statistical data. Moscow, RIO CNIIOIZ, 2017, 56 p. (In Russ.)
  6. Sadykov A.S., Agzamov A.M. Ways to improve treatment effectiveness in progressive fibro-cavernous pulmonary TB. In: Urgent issues of TB control: Proceedings of the Jubilee session dedicated to the 90th anniversary of the Central TB Research Institute. Moscow, 2011, pp. 159–161. (In Russ.)
  7. TB in the Russian Federation in 2011/2013/2014. The analytical review of statistical indicators used in the Russian Federation and worldwide. Moscow, 2015, p. 309. (In Russ.)
  8. Javorsky K.M. et al. Chronic forms of pulmonary TB and their influence on the epidemiological situation in the Republic of Moldova. In: Urgent issues of TB control: Proceedings of the Jubilee session dedicated to the 90th anniversary of the Central TB Research Institute. Moscow, 2011, pp. 225–226. (In Russ.)
  9. Ergeshov A.E., Punga V.V., Rusakova L.I., Yakimova M.A., Izmailova T.V. The epidemiological situation of TB in the Russian Federation. Nauka, novye tekhnologii i innovatsii Kyrgyzstana, 2017, no. 8, pp. 11–15. (In Russ.)
  10. Global tuberculosis report, 2017. Chapter 3. TB disease burden. WHO, 2017, pp. 21–63.
  11. Global tuberculosis report, 2015, WHO.
KEYWORDS:

fibro-cavernous pulmonary TB, early detection, preventive screening, surgical treatment.

FOR CORRESPONDENCE:

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

Marina A. Yakimova, Candidate of Medical Sciences, Leading Researcher, Research Administration

Department

Tel.: +7 (499) 785-90-79

E-mail: marina-air@mail.ru

Larisa I. Rusakova, Doctor of Medical Sciences, Principal Researcher, Head, Research Administration

Department

Tel.: +7 (499) 785-91-87

E-mail: larisa.rusakova@mail.ru

Svetlana S. Sadovnikova, Doctor of Medical Sciences, Head, Surgery no. 1

tel.: +7 (499) 748-30-14

E-mail: cniitramn@yandex.ru

Tamara V. Izmailova, Researcher, Research Administration Department

Tel.: +7 (499) 785-91-87

E-mail: cniitramn@yandex.ru

IMMUNOLOGICAL ASPECTS OF INTOLERANCE OF ANTITUBERCULOSIS DRUGS

Article 9.Page 65.
ARTICLE TITLE:

IMMUNOLOGICAL ASPECTS OF INTOLERANCE OF ANTITUBERCULOSIS DRUGS

DOI: 10.7868/S2587667819030099

AUTORS:

Aberbakh M.M., Gergert V.Ya.

Central TB Research Institute, Moscow, Russia

DESCRIPTION OF ARTICLE:

Submitted as of 18.01.2019

We review the current classifications of adverse events induced by administration of antituberculosis drugs. Immunologically mediated adverse events, allergies or delayed-type hypersensitivity reactions, account for the smallest share of all druginduced reactions. Based on the chemical structure of drugs, adverse reactions are divided into immunoglobulin E-mediated reactions, immunoglobulin G3-mediated reactions, immunocomplex-mediated reactions, and T-cell-mediated reactions. The latter type reactions are divided into subtypes: IVa, IVb, IVc, and IVd, based on T-cell phenotypes involved in pathological processes. The humoral factors involved in effector mechanisms, which influence target organs, are well-known. We review the current data on immunological reactions induced by administration of antituberculosis drugs and possible laboratory methods of their identification. We also provide the major results of researches into immunological indicators of the blasttransformation reaction to cell culture stimulation with isoniazid, rifampicin, ethambutol, and pyrazinamide.

The research was performed under scientific theme № 0515-2019-0016 “Personalized approaches to the treatment of respiratory TB in children and adolescents”.

REFERENCES:
  1. Dubrovskaya N.A. Clinical and immunological manifestations of rifampicin-induced adverse reactions in TB treatment. Thesis for a Candidate’s degree in Medicine. Moscow, 1984 (In Russ.)
  2. Klyuchnikov S.O. Polypragmasy: ways of solution. Detskie infectsii, 2014, no. 4, pp. 36–41. (In Russ.)
  3. Litvinova L.S., Gutsol A.A., Sokhanevich N.A., Kofanova K.A., Khaziakhmatova O.G., Shchupletsova V.V., Kaigorodova E.V., Goncharov A.G. Major surface markers of functional activity of T-lymphocytes. Medicinskaya immunologia, 2014, vol. 16, no. 1, pp. 7–26. (In Russ.)
  4. Maslauskene T.P., Nikolaeva S.V. Side-effects of antituberculosis drugs. Sibirsky medicinsky jurnal, 2005, vol. 52, no. 3, pp. 13–19. (In Russ.)
  5. Mellon M., Schatz M., Patterson R. Drug allergy. In: Clinical immunology and allergology. Ed. by Lolor-Jr., T. Fisher, D. Adelman. Translated from English. Moscow, Praktika, 2000, pp. 357–365. (In Russ.)
  6. Novikov P.D. Clinical immunopathology. Moscow, 2009. (In Russ.)
  7. Baecher-Allan C., Brown J.A., Freeman G.J., Hafler D.A. CD4+CD25 high regulatory cells in human peripheral blood. J. Immunol, 2001, vol. 167, no. 3, pp. 1245–1253.
  8. Chatila T.A. Role of regulatory T-cells in human diseases J. Allergy Clin Immunol, 2005, vol. 116, no. 5, 949–959.
  9. Chen R., Zhang Y., Tang S., Lu X., Wu S., Sun F., Xia Y., Zhan S.Y. The association between HLA-DQB1 polymorphism and antituberculosis drug induced liver injury: a case–control study. Journal of Clinical Pharmacy and Therapeutics, 2014. doi: 10.1111/jcpt.12211.
  10. Clausen J., Vergeiner B., Enk M., Petzer A.L., Gastl G., Gunsilius E. Functional significance of the activationassociated receptor СD25 and CD69 on human NK-cells and NK-like T-cells. Immunobiology, 2003, vol. 207, no. 2, pp. 85–93.
  11. Coombs P.R., Gell P.G. Classification of allergic reactions responsible for clinical hypersensitivity and disease. In: Gell R.R., Еd. Clinical aspects of immunology. Oxford, Oxford University Press, 1968, pp. 575–596.
  12. Gaffen S.L. Signaling domains of the interleukin‑2 receptor. Cytokine, 2001, vol. 14, no. 2, pp. 63–77.
  13. Gerosa F., Tommasi M., Scardoni M., Accolla R.S., Pozzan T., Libonati M., Tridente G., Carra G. Structural analysis of the CD69 early activation antigen by two monoclonal antibodies directed to different epitopes. Mol Immunol., 1991, vol. 28, no. 1–2, pp. 159–168.
  14. Grossman W.J., Verbsky J.W., Barchet W., Colonna M., Atkinson J.P., Ley T.J. Human T-regulatory cells can use the perforin pathway to cause autologous target cell death. Immunity, 2004, vol. 21, no. 4, pp. 589–601.
  15. Gomes E.R., Demoly P. Epidemiology of hypersensitivity drug reactions. Curr Opin Allergy Clin Immunol, 2005, vol. 5, pp.309–316.
  16. Khan S., Andries A., Pherwani A., Saranchuk P., Isaakidis P. Patch-testing for the management of hypersensitivity reactions to second-line antituberculosis drugs: a case report. BMC Research Notes,  2014, 7:537 http://www.biomedcentral.com/1756-0500/7/537.
  17. Kim S-H., Lee S-L., Kim S-H., Park H-W., Chang Y-S., Lee K.W., Jee Y-K. Antituberculosis drug-induced hypersensitivity syndrome and its association with human leukocyte antigen. Tuberculosis, 2013, vol. 93, 270–274.
  18. KoyanagiM., Kawakabe S., Arimura Y. A comparative study of colorimetric cell proliferation assays in immune cells. Cytotechnology, 2016, vol. 68, pp.1489–1498.
  19. Lin J., Weiss A. T cell receptor signaling. J Cell Sci, 2001, vol. 114, no. 2, pp. 243–244.
  20. Lochmatter P., Zawodniak A., Pichler W.J. In vitro tests in drug hypersensitivity diagnosis. Immunol Allergy Clin N Am, 2009, vol. 29, pp. 537–554, doi:10.1016/j. iac.2009.04.009.
  21. Mayorga C., Sanz M.L., Gamboa P.M., Garcı´a B.E., Labrador M., Lahoz C., Longo Areso N., Lo´ pez Hoyos M., Martı´nez Quesada J., Monteseirı´n F.J. In vitro diagnosis of immediate allergic reactions to drugs: an update. J Investig Allergol Clin Immunol, 2010, vol. 20, 103–109.
  22. Munsaka S.M., Agsalda M., Troelstrup D., Hu N., Yu Q., Shiramizu B. Characteristics of activated monocyte phenotype support R5-tropic human immunodeficiency virus. Immunol Immunogenet Insights, 2009, vol. 1, pp. 15–20.
  23. Nyfeler B., Pichler W.J. The lymphocyte transformation test for the diagnosis of drug allergy: sensitivity and specificity. Clin Exp Allergy, 1997, vol. 27, pp. 175–181.
  24. Pichler W.J., Tilch J. The lymphocyte transformation test in the diagnosis of drug hypersensitivity. Allergy, 2004, vol. 59, pp. 809–820.
  25. Rawlins M., Thompson W. Mechanisms of adverse drug reactions. In: Davies D., ed. Textbook of adverse drug reactions. New York, Oxford University Press, 1991, pp. 18–45.
  26. Riss T.L., Moravec R.A., Niles A.L., Benink H.A., Worzella T.J., Minor L. Cell viability assays. In: Sittampalam G.S., Coussens N.P., Nelson H. (ed) Assay guidance manual, 2013. http://www.ncbi.nlm.nih.gov/books/NBK144065/.
  27. Shipkova M., Wieland E. Surface markers of lymphocyte activation and markers of cell proliferation. Clinica Chimica Acta, 2012, vol. 413, pp. 1338–1349.
  28. Scholzen T., Gerdes J. The Ki‑67 protein: from the known and the unknown. J. Cell. Physiol., 2000, vol. 182, no. 3, pp. 311–322.
  29. Sun Q., Sha W., Gui X-W., Xiao Y-J., Zeng W-H., Sun W-W., Xiao H-P., Ye W-Y. Drug-induced lymphocyte stimulation test in the prediction of druginduced hypersensitivity to antituberculosis drugs. Diagn Microbiol Infect Dis, 2015. http://dx.doi.org/10.1016/j.diagmicrobio.2015.03.008.
  30. Suzuki Y., Miwa S., Shirai M., Ohba H., Murakami M., Fujita K. Suda T., Nakamura H., Hayakawa H., Chida K. Drug lymphocyte stimulation test in the diagnosis of adverse reactions to antituberculosis drugs. Chest, 2008, vol. 134, pp. 1027–1032.
  31. Treatment of tuberculosis: guidelines – 4th ed. WHO/HTM/TB/2009.420. ISBN978 92 4454783 0
  32. Umeki S. Adverse effects of antitubercular drugs and significance of measurement of the drug-stimulating lymphocyte transformation rate. Jpn J Med, 1989, vol. 28, no. 3, pp. 335–340.
  33. WHO. International drug monitoring: the role of national centres. Tech Rep Ser WHO, no. 498, 1972.
KEYWORDS:

antituberculosis drugs, immunopathological adverse events.

FOR CORRESPONDENCE:

Central ТВ Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

Mikhail M. Averbakh, Doctor of Medical Sciences, Professor, Principal Researcher, Immunology Department

Tel.: +7 (499) 780-49-97

E-mail: amm50@mail.ru

Vladislav Ya. Gergert, Doctor of Medical Sciences, Professor, Principal Researcher, Head of Immunology

Department

Tel.: +7 (499) 785-90-72

E-mail: hergertv@mail.ru

DETECTION OF MYCOBACTERIA BY FLUORESCENT MICROSCOPY

PART 1. SPECIMEN PREPARATION AND STAINING

Article 10.Page 74.
ARTICLE TITLE:

DETECTION OF MYCOBACTERIA BY FLUORESCENT MICROSCOPY

PART 1. SPECIMEN PREPARATION AND STAINING

DOI: 10.7868/S2587667819030105

AUTORS:

Sevastyanova E.V., Larionova E.E., Andrievskaya I.Yu.

Central TB Research Institute, Moscow, Russia

DESCRIPTION OF ARTICLE:

Submitted as of 19.06.2019

The article presents a method of mycobacteria (MB) detection by microscopy examination of specimens stained with fluorescent method; describes options for specimen preparation from sediment of the diagnostic material for culture examinations and from the sediment of untreated liquid specimens; presents two methods of staining with fluorescent dyes. The article also provides an overview of possible errors in smear preparation and staining for fluorescent microscopy.

REFERENCES:
  1. Chernousova L.N., Sevastyanova E.V., Andreevskaya S.N., Larionova E.E., Smirnova T.G., Erokhin V.V., Algorithm of microbiological examinations for the diagnosis of tuberculosis infection: Handbook for Doctors.
  2. UMO‑17–28/248 of 12.07.11, M, 2011, p. 49. (In Russ.)
  3. Golyshevskaya V.I., Sevastyanova E.V., Shulgina M.V., Evguschenko G.V., TB Detection by Microscopy Method. Textbook for the Basic Training Course «TB Detection by the Microscopy Method» UMO‑107, 08.02.07), M., Tver, LLC «Publishing house «Triada», 2008, p. 100. (In Russ.)
  4. Executive Order no. 109 of the Russian Ministry of Health of 21 March 2003 «On Improvement of Antituberculosis Measures in the Russian Federation», Annex 11 «Guideline on the Unified Methods of Microbiological Examinations in TB Detection, Diagnosis and Treatment». (In Russ.)
  5. Golyshevskaya V.I., Shulgina M.V., Sevastyanova E.V. Akimkin V.G., Vanina G.M., Vakhrusheva D.V., Vishnevsky B.I., Vladimirsky M.A., Irtuganova O.A., Kravchenko M.A., Otten T.F., Popov S.A., Safonova S.G., Fedorova L.S. Culture methods for TB diagnosis: Manual for the Basic Training of TB Bacteriological Laboratory Specialists, EMA‑685, 24.10.07, under the editorship of the RAMS corresponding member prof. V.V. Erokhin, M., Tver, LLC «Publishing house «Triada», 2008, p. 208. (In Russ.)
  6. Golyshevskaya V.I., Egorova O.V. Sevastyanova E.V., Shulgina M.V. Fluorescent Microscopy, Textbook for Training Courses: Culture Methods of TB Diagnosis, Microscopy Method for TB Detection, UMO‑691, 24.10.07, M., Tver: LLC Publishing house Triada, 2008, p. 36. (In Russ.)
  7. Sevastyanova E.V., Larionova E.E., Andrievskaya I.Yu., Identification of Mycobacteria by Ziehl–Nielsen Smears Microscopy Method. Part 1. Preparation and Staining of Specimens for Microscopy, CTRI Bulletin, 2019, no. 1, pp. 100–108. DOI: 10.7868/S25876678190114 (In Russ.)
  8. Chernousova L.N., Sevastyanova E.V., Larionova E.E., Smirnova T.G., Andreevskaya S.N., Popov S.A., Zhuravlev V.Yu., Puzanov V.A., Mariandyshev A.O., Vakhrusheva D.V., Kravchenko M.A., Safonova S.G., Vasilyeva I.A., Ergeshov A.E. Federal Clinical Recommendations for Management and Implementation of Microbiological and Molecular Genetic Diagnosis of Tuberculosis, RSP, Moscow, 2015, p. 35. (In Russ.)
KEYWORDS:

mycobacteria, fluorescent microscopy method.

FOR CORRESPONDENCE:

Central TB Research Institute

2, Yauzskaya alley, 107564, Moscow, Russia

Elina V. Sevastyanova, Doctor of Biological Science, Leading Researcher, Department of Microbiology

Tel: +7 (499) 785-90-91

E-mail: elinasev@yandex.ru

Elena E. Larionova, Candidate of Biological Science, Senior Researcher, Department of Microbiology

Tel: +7 (499) 785-90-91

E-mail: larionova_lena@mail.ru

Irina Yu. Andrievskaya, Junior Researcher, Department of Microbiology

Tel: +7 (499) 785-90-91

E-mail: andrievskaya.iri@mail.ru